Protein Info for MPMX19_01911 in Azospirillum sp. SherDot2

Annotation: Acetoacetyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 240 TIGR01829: acetoacetyl-CoA reductase" amino acids 3 to 239 (237 residues), 354.2 bits, see alignment E=1.9e-110 PF00106: adh_short" amino acids 3 to 189 (187 residues), 195.7 bits, see alignment E=1.5e-61 PF08659: KR" amino acids 6 to 177 (172 residues), 61.3 bits, see alignment E=3e-20 PF01370: Epimerase" amino acids 6 to 178 (173 residues), 26.4 bits, see alignment E=1.1e-09 PF13561: adh_short_C2" amino acids 12 to 238 (227 residues), 205.9 bits, see alignment E=1.7e-64 PF23441: SDR" amino acids 147 to 237 (91 residues), 30.4 bits, see alignment E=6.5e-11

Best Hits

Swiss-Prot: 66% identical to PHAB_RHIME: Acetoacetyl-CoA reductase (phaB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00023, acetoacetyl-CoA reductase [EC: 1.1.1.36] (inferred from 97% identity to azl:AZL_023120)

MetaCyc: 66% identical to (R)-3-hydroxybutyryl-CoA dehydrogenase (Cereibacter sphaeroides)
Acetoacetyl-CoA reductase. [EC: 1.1.1.36]

Predicted SEED Role

"Acetoacetyl-CoA reductase (EC 1.1.1.36)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 1.1.1.36)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (240 amino acids)

>MPMX19_01911 Acetoacetyl-CoA reductase (Azospirillum sp. SherDot2)
MARVAVVTGGTRGIGEAVAVALKNAGYKVAANYAGNDQAAEEFTARTGIPTYKFDVADFD
ACKKGIAAIESDLGPVDVLINNAGITRDGVMHRMTYDQWEKVIHTNLSSCFNMCRNVIDG
MRERGFGRIVNIGSVNGQAGQYGQVNYAAAKSGIHGFTKALAQESAAKGITVNAIAPGYI
DTDMVRAVPANVLEKIVARIPVGRLGRASEIAKAVLYLVDDDNGFMTGSTLSVNGGQHMY