Protein Info for MPMX19_01910 in Azospirillum sp. SherDot2

Annotation: 3-octaprenyl-4-hydroxybenzoate carboxy-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00148: decarboxylase, UbiD family
  • amino acids 6 to 462 (457 residues), 534 bits, E=1.2e-164
• PF20695: UbiD_N
  • amino acids 10 to 91 (82 residues), 84.6 bits, E=6.2e-28
• PF01977: UbiD
  • amino acids 126 to 329 (204 residues), 277.9 bits, E=5.5e-87
• PF20696: UbiD_C
  • amino acids 335 to 459 (125 residues), 167.4 bits, E=2.2e-53

Best Hits

Swiss-Prot: 61% identical to Y821_RICPR: Uncharacterized protein RP821 (RP821) from Rickettsia prowazekii (strain Madrid E)

KEGG orthology group: K03182, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiD [EC: 4.1.1.-] (inferred from 99% identity to azl:AZL_023110)

MetaCyc: 64% identical to 3-octaprenyl-4-hydroxybenzoate decarboxylase (Escherichia coli K-12 substr. MG1655)
3-OCTAPRENYL-4-OHBENZOATE-DECARBOX-RXN [EC: 4.1.1.98]

Predicted SEED Role

"3-polyprenyl-4-hydroxybenzoate carboxy-lyase (EC 4.1.1.-)" in subsystem Ubiquinone Biosynthesis (EC 4.1.1.-)

MetaCyc Pathways

• superpathway of ubiquinol-8 biosynthesis (early decarboxylation) (7/12 steps found)
• ubiquinol-10 biosynthesis (early decarboxylation) (4/8 steps found)
• ubiquinol-8 biosynthesis (early decarboxylation) (4/8 steps found)
• plastoquinol-9 biosynthesis II (1/5 steps found)
• ubiquinol-9 biosynthesis (early decarboxylation) (3/8 steps found)
• ubiquinol-7 biosynthesis (early decarboxylation) (2/8 steps found)
• superpathway of chorismate metabolism (38/59 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Ascorbate and aldarate metabolism
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Fluorene degradation
• Phenylalanine metabolism
• Phenylpropanoid biosynthesis
• Purine metabolism
• Pyruvate metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.-

Use Curated BLAST to search for 4.1.1.- or 4.1.1.98

Protein Sequence (504 amino acids)

>MPMX19_01910 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (Azospirillum sp. SherDot2)
MPYTSLRDFIDRLEKAGRLVVVKEPVSSVLEMTEIQTRLLAENGPAVLFENVIKADGTRS
EMPVLVNLFGTVERVAWGMDREPHQLRAVGETLAFLKQPEPPGGFREALDLIPLAKTVLS
MKPKTVGSAPCQEVVLTGDDIDLGRLPVQSCWPGEPAPLITWPLVVTKGPTGKREDDFNL
GIYRMQVLGKNKTIMRWLKHRGGAQHHHRWAASGKREPLPCAVVLGADPGTILAAVTPVP
DTLSEYQFAGLLRGKKVELVECKTVPLKVPAQAEIVLEGHVSLDEYADEGPYGDHTGYYN
SVEPFPVFTVTAMTMRRKPIYLSTFTGRPPDEPSVLGEALNEVFIPLLIQQFPEIVDFWL
PPEGCSYRIAVVSMKKAYPGHAKRVMMGVWSFLRQFMYTKWVIVVDDDIDARNWKDVMWA
ISTRMDPARDITVIEGTPIDYLDFASPESGLGGKVGLDATNKWPPETKREWGEKLHMTDE
VVEAVSRKWDLYGLPGSGKPIWKK