Protein Info for MPMX19_01842 in Azospirillum sp. SherDot2

Annotation: FeMo cofactor biosynthesis protein NifB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 TIGR01290: nitrogenase cofactor biosynthesis protein NifB" amino acids 46 to 487 (442 residues), 667.6 bits, see alignment E=4e-205 PF04055: Radical_SAM" amino acids 75 to 254 (180 residues), 67.2 bits, see alignment E=2e-22 PF02579: Nitro_FeMo-Co" amino acids 393 to 485 (93 residues), 76.1 bits, see alignment E=2.1e-25

Best Hits

Swiss-Prot: 70% identical to NIFB_HERSE: FeMo cofactor biosynthesis protein NifB (nifB) from Herbaspirillum seropedicae

KEGG orthology group: K02585, nitrogen fixation protein NifB (inferred from 96% identity to azl:AZL_022470)

Predicted SEED Role

"Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (520 amino acids)

>MPMX19_01842 FeMo cofactor biosynthesis protein NifB (Azospirillum sp. SherDot2)
MSNVVSLDSIFGLGELAPPAMTPAAPEAASGCSSSSCGSSDGPADMDPAVWEKVKNHPCY
SEEAHHYFARMHVAVAPACNIQCNYCNRKYDCSNESRPGVVSEKLTPDQALKKILAVAQE
IPQLSVIGIAGPGDSLAAAGKNTFATFEMLQKKAPDLKLCLSTNGLALPDHVDTIAQYNI
DHVTITINMVDPEVGAQIYPWIFYKHKRWTGLDAAKILHEQQMLGLEMLTSRGILVKVNS
VMIPGVNDEHLLEVNKAVKSRGAFLHNIMPLISDPAHGTWFGLNGQRGPTAQELKVVQDA
CEGGAKLMRHCRQCRADAVGLLGEDRGEEFTADKIEAMGIVDYDDEARRTYREHVESERA
GRNASKAAAQADVRESIGLTVDPILIAVATKGGERINEHFGHAKEFQIYEVGPAGAKFVG
HRRVDQYCEGGSGDVDTLDGVLAAINDCTAVFVAKIGGCPSKSLADAGIEPVDRFAFDYI
EESALAYFKDYAERLGQGVVQSRAGQDAVIRTGALTARRA