Protein Info for MPMX19_01840 in Azospirillum sp. SherDot2

Annotation: Nif-specific regulatory protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 TIGR01817: Nif-specific regulatory protein" amino acids 14 to 467 (454 residues), 586.6 bits, see alignment E=2.6e-180 PF13185: GAF_2" amino acids 29 to 173 (145 residues), 53.6 bits, see alignment E=1.3e-17 PF01590: GAF" amino acids 30 to 172 (143 residues), 60.7 bits, see alignment E=1e-19 PF00158: Sigma54_activat" amino acids 208 to 374 (167 residues), 244 bits, see alignment E=3e-76 PF14532: Sigma54_activ_2" amino acids 210 to 378 (169 residues), 76.2 bits, see alignment E=1.4e-24 PF07724: AAA_2" amino acids 229 to 354 (126 residues), 28.8 bits, see alignment E=5.4e-10 PF07728: AAA_5" amino acids 231 to 349 (119 residues), 27.3 bits, see alignment E=1.5e-09 PF00004: AAA" amino acids 231 to 351 (121 residues), 24.8 bits, see alignment E=1.2e-08 PF02954: HTH_8" amino acids 595 to 629 (35 residues), 38.3 bits, see alignment (E = 4e-13)

Best Hits

Swiss-Prot: 82% identical to NIFA_AZOBR: Nif-specific regulatory protein (nifA) from Azospirillum brasilense

KEGG orthology group: K02584, Nif-specific regulatory protein (inferred from 91% identity to azl:AZL_022450)

Predicted SEED Role

"Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (636 amino acids)

>MPMX19_01840 Nif-specific regulatory protein (Azospirillum sp. SherDot2)
MPSAAQSASSSSLELLTIYEVSKILGSSLDLEQTLREVLRALSYQLQMHRGRVYLQGEDG
ALRLVAAQGLPKDSPAQEIDYNQGEGISGRILKTGMPAVVPNLAEEPLFNNRSGGRDDLD
EQVASLVGVPIKAAGAVIGVLTIDRISDDGPSGHFGSDVRFLTMVANLIGQTVRLHRTVA
EERRFMMRETFRVQKELRPVVAPVNDVVCTSPNMVDVLAQVHRVAPFKSTVLIRGESGTG
KELIARAIHNLSPRKEAPFIRVNCAALPESLLESELFGHEKGSFTGAQKDHKGRFELASG
GTLFLDEIGDISSNFQGKLLRVLQEQEFERVGGSKTIKTDVRLICATNLNLEEAVGHGKF
RADLYFRINVVTIHLPPLRERRGDIAMLAKHFVGKFGRDNGLNLDIAQDALEVLNRCTWP
GNVRELENCIERAATQCRNGTIRVPDLSCSMNLCNSSVLFQYRTMGAAVGGLAPSMSGTP
TNPSVAPPAPGRPQTPAMPPAMPAPANGGGHGAVNGAPSGAPNGAQWPACASGCGAGPMP
ACGASKPLPPSAIVPLPTPQPAASAPAVPSAPAPQPASAGPDLPLDEPESGPLRDRLIWA
MERTGWVQAKAARLLGMTTRQVSYALRKYNIEIKRF