Protein Info for MPMX19_01823 in Azospirillum sp. SherDot2

Annotation: ADP-L-glycero-D-manno-heptose-6-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR02197: ADP-glyceromanno-heptose 6-epimerase" amino acids 2 to 322 (321 residues), 392.2 bits, see alignment E=8.7e-122 PF04321: RmlD_sub_bind" amino acids 2 to 157 (156 residues), 32.5 bits, see alignment E=1.3e-11 PF01370: Epimerase" amino acids 2 to 249 (248 residues), 143 bits, see alignment E=2.7e-45 PF01073: 3Beta_HSD" amino acids 3 to 154 (152 residues), 35.4 bits, see alignment E=1.5e-12 PF16363: GDP_Man_Dehyd" amino acids 4 to 270 (267 residues), 58.6 bits, see alignment E=1.9e-19

Best Hits

KEGG orthology group: K03274, ADP-L-glycero-D-manno-heptose 6-epimerase [EC: 5.1.3.20] (inferred from 95% identity to azl:AZL_022310)

Predicted SEED Role

"ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20)" in subsystem LOS core oligosaccharide biosynthesis (EC 5.1.3.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (328 amino acids)

>MPMX19_01823 ADP-L-glycero-D-manno-heptose-6-epimerase (Azospirillum sp. SherDot2)
MIVVTGGAGFIGSNLVAALAARGITDVAVVDRFREGEKWQNLAKREVAALVPPEQTLAFL
DQHAAEIDTVFHLGAISATTERDVDKIVANNVALTLDLWRWCSWRGCRLVYASSAATYGD
GAVGFDDDGSCDGLARLRPLNAYGWSKHLVDRRIARAVAGGDLVPPQWAGLKFFNVYGPN
EAHKGDMMSVIAKLHPQLMAGQPARLFKSHKDGFEDGGQLRDFIHVDDCVAVMLWLYDHP
DVSGLFNVGTGKARSFKDLALATFAALGLPPRIEYFEMPEHLRGKYQYFTQATTDRLRAA
GFDQPFTELEEGVRRYVQDFLSQPDPYR