Protein Info for MPMX19_01724 in Azospirillum sp. SherDot2

Annotation: Proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 74% identical to SYP_PARL1: Proline--tRNA ligase (proS) from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 98% identity to azl:AZL_021250)

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

tRNA charging (19/21 steps found)
pyoluteorin biosynthesis (1/7 steps found)
ergotamine biosynthesis (4/12 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
clorobiocin biosynthesis (1/10 steps found)
superpathway of ergotamine biosynthesis (5/18 steps found)
pyrrolomycin biosynthesis (1/14 steps found)
sulfazecin biosynthesis (1/16 steps found)
phosalacine biosynthesis (4/25 steps found)
phosphinothricin tripeptide biosynthesis (4/25 steps found)
colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (437 amino acids)

>MPMX19_01724 Proline--tRNA ligase (Azospirillum sp. SherDot2)
MRLSSFFMPTLKETPTEAQIVSHRLMLRAGMIRQTSAGIYAWLPLGYRVLRKIEQIVREE
QDAAGAQELLMPTIQSADLWRESGRYDDYGKEMLRITDRHDREMLFGPTNEEMITDIFRS
FVKSYRQLPLNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDIDAAGARRSYQKMFLA
YLRTFARIGLKAIPMRADTGPIGGDLSHEFIILAETGESGVFCHKDWMNLDVLKDAPGME
DDLQPFFDRITSIYAATDEKHDPANSPVPEADLVSARGIEVGHIFNFGTKYSKPMNAVVT
GPNGESIPVEMGSYGIGVSRLMGAIIEASHDDNGIIWPDAVAPFNVGLINLKSGDAETDR
VCAELYAKLESAGLEVAYDDRDERPGAKFADMDLIGVPWQLVVGPRGLKNGVVELKRRAT
DEKEELPVEAALAKLLG