Protein Info for MPMX19_01675 in Azospirillum sp. SherDot2

Annotation: Phospho-N-acetylmuramoyl-pentapeptide- transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 transmembrane" amino acids 22 to 42 (21 residues), see Phobius details amino acids 74 to 91 (18 residues), see Phobius details amino acids 97 to 115 (19 residues), see Phobius details amino acids 135 to 154 (20 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 200 to 220 (21 residues), see Phobius details amino acids 240 to 257 (18 residues), see Phobius details amino acids 264 to 283 (20 residues), see Phobius details amino acids 289 to 312 (24 residues), see Phobius details amino acids 339 to 358 (20 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 39 to 361 (323 residues), 432.6 bits, see alignment E=5.3e-134 PF10555: MraY_sig1" amino acids 69 to 81 (13 residues), 21.9 bits, see alignment (E = 8.9e-09) PF00953: Glycos_transf_4" amino acids 100 to 284 (185 residues), 108.3 bits, see alignment E=4e-35

Best Hits

Swiss-Prot: 80% identical to MRAY_RHOCS: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 98% identity to azl:AZL_020790)

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (361 amino acids)

>MPMX19_01675 Phospho-N-acetylmuramoyl-pentapeptide- transferase (Azospirillum sp. SherDot2)
MLYNLLFGLADVFSPFNLFRYLTFRTGGAVITSLVISFVFFPRLIAWLKRKQGEGQPIRA
DGPESHFKKKGTPTMGGLMILIAMTVSTLLWADLTNAYIWIVLLVTIGYGLIGFGDDYLK
LTKRNTKGLSGRFRLGWEIAIGLVASALIMWVSAPPLSGGVAVPFVKDFLVQLSWFFVPF
GAFIMVGASNSVNLTDGLDGLAIVPTMIAAGCFGLISYLSGNAIFANYLQIHHVPGSGEL
AVFCGALVGAGLGFLWYNAPPAMVFMGDTGSLSLGGALGAVSVVTKHEIVLAIIGGLFVL
ETVSVIVQVASFKLTGKRVFRMAPLHHHFEKKGWAEPTVVIRFWIIASILALVGLSTLKL
R