Protein Info for MPMX19_01548 in Azospirillum sp. SherDot2

Annotation: ATP-dependent Clp protease proteolytic subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 208 transmembrane" amino acids 37 to 58 (22 residues), see Phobius details amino acids 96 to 116 (21 residues), see Phobius details TIGR00493: ATP-dependent Clp endopeptidase, proteolytic subunit ClpP" amino acids 10 to 200 (191 residues), 324.8 bits, see alignment E=9.2e-102 PF00574: CLP_protease" amino acids 21 to 200 (180 residues), 293 bits, see alignment E=4.6e-92

Best Hits

Swiss-Prot: 97% identical to CLPP_AZOBR: ATP-dependent Clp protease proteolytic subunit (clpP) from Azospirillum brasilense

KEGG orthology group: K01358, ATP-dependent Clp protease, protease subunit [EC: 3.4.21.92] (inferred from 99% identity to azl:AZL_019170)

MetaCyc: 65% identical to ATP-dependent Clp protease proteolytic subunit (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92)" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent or cAMP signaling in bacteria (EC 3.4.21.92)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (208 amino acids)

>MPMX19_01548 ATP-dependent Clp protease proteolytic subunit (Azospirillum sp. SherDot2)
MYDFEPKMNALVPMVIEQTNRGERAYDIYSRLLKERIIFLIGGVNDAVASLICSQLLFLE
SENPNKDIAIYINSPGGYVSAGLAIYDTMQYIRPQVSTVCMGQAASMGSLLLAAGAPGKR
FSLPNSRIMIHQPSGGAQGQASDIEIQAQEILKLRSRLNDIYVKHTGQDLDTIETAMERD
KFMSPEEAKAFGLIDEVVEKRPGVDHHS