Protein Info for MPMX19_01468 in Azospirillum sp. SherDot2

Annotation: Methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 PF00133: tRNA-synt_1" amino acids 5 to 65 (61 residues), 35.5 bits, see alignment E=1e-12 amino acids 225 to 337 (113 residues), 58.7 bits, see alignment E=9.5e-20 TIGR00398: methionine--tRNA ligase" amino acids 7 to 478 (472 residues), 524.4 bits, see alignment E=1.8e-161 PF09334: tRNA-synt_1g" amino acids 8 to 365 (358 residues), 371.6 bits, see alignment E=8.6e-115 PF01406: tRNA-synt_1e" amino acids 16 to 139 (124 residues), 38.2 bits, see alignment E=2.3e-13 amino acids 258 to 345 (88 residues), 29.5 bits, see alignment E=1e-10 PF19303: Anticodon_3" amino acids 376 to 509 (134 residues), 50 bits, see alignment E=6.3e-17

Best Hits

Swiss-Prot: 61% identical to SYM_BRUSU: Methionine--tRNA ligase (metG) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 96% identity to azl:AZL_018180)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (516 amino acids)

>MPMX19_01468 Methionine--tRNA ligase (Azospirillum sp. SherDot2)
MAGPKTFYLTTPIYYVNDVPHIGHAYTTLACDVLARFMRLDGYDVKFLTGTDEHGQKVEK
SAEKAGIAPQEFTDRVSQNFRDLVSLMNYSNDDFIRTTEPRHVKSVQALWTVLKDRGEIY
LGSYAGWYSVRDEAFYGEDELTTNAEGKKIAPTGAECEWVEEPSYFFKLSAWQDRLIEFY
EQNPDFIAPAGKRNEVMSFVKSGLKDLSVSRTTFKWGIPVPGDDAHIMYVWLDALANYIT
AVGYPNTTGDYAKYWPADLHMVGKDILRFHAVYWPAFLMAAGLQPPRRVFAHGWWTIEGQ
KMSKSLGNVIAPDSLVNTYGLDQTRYFLLREVPFGNDGDFSHKQMVNRINGNLANDYGNL
VQRVLSMIGKNCGAAVPTPGAFTEADNALLGSAYGMLDIVRAEIKAQAFHKALDAIWAVV
GDANRYVDEQAPWALKKTDPARMATVLYVLAETIRHLAILTQPVMPDASAKILDLLALGP
DARGFGTLGPSGTLAAGTPLPTPQGVFPRYVEEPKV