Protein Info for MPMX19_01192 in Azospirillum sp. SherDot2

Annotation: Peptide chain release factor 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 TIGR00503: peptide chain release factor 3" amino acids 1 to 526 (526 residues), 704.7 bits, see alignment E=6.3e-216 PF00009: GTP_EFTU" amino acids 10 to 275 (266 residues), 169.9 bits, see alignment E=9.5e-54 TIGR00231: small GTP-binding protein domain" amino acids 14 to 146 (133 residues), 71.1 bits, see alignment E=9.4e-24 PF01926: MMR_HSR1" amino acids 14 to 141 (128 residues), 26.3 bits, see alignment E=1.4e-09 PF22042: EF-G_D2" amino acids 293 to 379 (87 residues), 79.8 bits, see alignment E=2.7e-26 PF16658: RF3_C" amino acids 385 to 512 (128 residues), 147.7 bits, see alignment E=3.4e-47

Best Hits

Swiss-Prot: 57% identical to RF3_METCA: Peptide chain release factor 3 (prfC) from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 99% identity to azl:AZL_014230)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (535 amino acids)

>MPMX19_01192 Peptide chain release factor 3 (Azospirillum sp. SherDot2)
MSELQDAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQMAGAVKARGEQRRAKSDWMKV
ERERGISVTASVMTFDFDGRTFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDGAKGIETQ
TLKLFEVCRLRDVPIMTFCNKMDREARDPFDLISEIESALALEVTPASWPIGMGRDFLGC
YDLIRDRLILMDRSKGDEIDDGIECNGLDDPRLDELLPAHAVAKLREEVEMARGLMPAFD
LEAYRAGHLTPIYFGSAINNFGVRELLSGLAENAPPPRPQPADPRTVEPDEEKVTGFVFK
VQANMDPNHRDRIAFVRLCSGRYKRGAKLKHIRSGKLMAVNNAVLFLARDREVAEEAWPG
DIMGIPNHGSLRIGDTLTEGEDLRFTGVPSFAPELLQRVRPDDPMRVKHLRKALEHFAEE
GASQVFKPLTGADWVVGVVGQLQFEVLASRIEAEYGIAARFEGAGLDAARWVETDDKVQL
EKFIELNRSALAEDHDGALVFLARNAWHLNRAQEDFPALRFLKTREQNRKVHTAA