Protein Info for MPMX19_01113 in Azospirillum sp. SherDot2

Annotation: Adaptive-response sensory-kinase SasA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 772 transmembrane" amino acids 32 to 51 (20 residues), see Phobius details amino acids 65 to 87 (23 residues), see Phobius details amino acids 107 to 132 (26 residues), see Phobius details amino acids 303 to 325 (23 residues), see Phobius details PF19312: NtrY_N" amino acids 35 to 315 (281 residues), 166.8 bits, see alignment E=3e-52 PF00672: HAMP" amino acids 328 to 378 (51 residues), 46.4 bits, see alignment 1.6e-15 PF00989: PAS" amino acids 399 to 503 (105 residues), 28.4 bits, see alignment E=5.7e-10 PF08448: PAS_4" amino acids 405 to 506 (102 residues), 29.6 bits, see alignment E=2.8e-10 PF13426: PAS_9" amino acids 408 to 505 (98 residues), 29.8 bits, see alignment E=2.4e-10 PF00512: HisKA" amino acids 517 to 583 (67 residues), 42.5 bits, see alignment E=2.2e-14 PF02518: HATPase_c" amino acids 627 to 743 (117 residues), 75.9 bits, see alignment E=1.4e-24

Best Hits

Swiss-Prot: 77% identical to NTRY_AZOBR: Nitrogen regulation protein NtrY homolog from Azospirillum brasilense

KEGG orthology group: K13598, two-component system, NtrC family, nitrogen regulation sensor histidine kinase NtrY [EC: 2.7.13.3] (inferred from 95% identity to azl:AZL_015060)

Predicted SEED Role

"Nitrogen regulation protein NtrY (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (772 amino acids)

>MPMX19_01113 Adaptive-response sensory-kinase SasA (Azospirillum sp. SherDot2)
MPPNSDIAAAGQRPVWQKILQWSRRVGLGKRLAFALSLAALAAGFATYAAMTESAPFGQA
NPRTVTLLLTLDLAILLTLGVLIARRIVAILIGRRRGLAGSRLHTRLVLVFSVLAVAPAI
IMTVFSTLFFYVGVQSWFSDRVRTAVNESLVVASAYLHEHQQNIRADALAMANDLSQEAR
FSSDPERFEQVVATQAMLRTLSEAIVFNGTTGAIVARAGYTFTLEFEPIPEDKIQQARTG
EVALIVKESDDRVRALVSLDRVSDTFLYVGRMVEPRVLQHMASAEGAVKEYTALENSRGS
LQVTFTLIFLVVAMLLLLAAVWAGLNFATRLVRPISALIGAAERVRAGDLTVRVSEPPGE
DEFVLLSRAFNRMTTEIEGQRRELLSTNRLLDERRRFTETVLSGVSAGVLGVDADGVINL
PNLSAARLLGEEDPERMIGMSLVELLPDIGDLLENAPKKPGRVVQDQIQIRRPGKTTLTL
LVRIAAEMRDGTVRGYVVTFDDITELVSAQRKAAWADVARRIAHEIKNPLTPIQLSAERL
RRKYLKEISSDTEVFTMCTDTIVRQVDDIRRMVDEFSAFARMPQPVMKPVNINDLVRQAV
FLQSSAHSGKIRFDTRLPSGPLTVACDSRQISQALTNLLQNAADAIEGRTSPADGADLPP
GEVAIAVEDDGEKVVVTVQDNGKGLPGGEQRDRLTEPYVTTRAKGTGLGLAIVKKIMEDH
GGVLTLEDREGGPGARVTLVIPHPVTVANDAGGRDDRPAETGGEMRHEAHGA