Protein Info for MPMX19_00972 in Azospirillum sp. SherDot2

Annotation: DNA topoisomerase 4 subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 TIGR01055: DNA topoisomerase IV, B subunit" amino acids 14 to 652 (639 residues), 793 bits, see alignment E=1e-242 PF02518: HATPase_c" amino acids 41 to 182 (142 residues), 67.8 bits, see alignment E=2.2e-22 PF00204: DNA_gyraseB" amino acids 239 to 408 (170 residues), 147.2 bits, see alignment E=6.9e-47 PF01751: Toprim" amino acids 436 to 542 (107 residues), 64.9 bits, see alignment E=1.3e-21 PF00986: DNA_gyraseB_C" amino acids 579 to 649 (71 residues), 73.5 bits, see alignment E=2.5e-24

Best Hits

Swiss-Prot: 62% identical to PARE_CAUVC: DNA topoisomerase 4 subunit B (parE) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 98% identity to azl:AZL_016410)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (662 amino acids)

>MPMX19_00972 DNA topoisomerase 4 subunit B (Azospirillum sp. SherDot2)
MSDLFENTAPKADSYSAQDIEVLEGLEPVRRRPGMYIGGTDDRALHHLVAEVLDNAMDEA
VAGHASRIDLELTADYTVMVRDNGRGIPIDDHPKYPGKSALEVIMTTLHSGGKFSNKVYQ
TSGGLHGVGLSVVNALSDRLTVEIAREKQLYVQEYSRGLPQGPLAHRGNVNRRGTTIRFH
ADPEIFGETAHFEPHRLYRLARSKAYLYRGVEIRWSCDPALLSVDATTPASETLHFPGGL
QDYLNAALKDRKTLTRLPFAGALDFPNGQGRVEWAVAWPDDDEGFSHTYCNTVPTPQGGT
HEAGLRTALTRGLKAYGELTNNKRAGQVTSDDVMGDACVLLSVFIREPHFQGQTKERLVT
AEAQRLVETAVKDHFDHWLSGDPQSANGLLERLIEKAEERARRKQSKEMTRKSATRRLRL
PGKLADCSRNSPEGTELFLVEGDSAGGSAKQARQRETQAILPLRGKILNVASASVDKMKA
NQELNDLTQALGCGIGKDFSTDKLRYERVIIMTDADVDGAHIASLLMTFFYREMPGLIQE
GHLYLALPPLYRLSHGNKQVYARDDAHKDELLSKMFKGKKPDISRFKGLGEMMPAQLRDT
TMDPSKRSLLRVVVPNAADPEEKPECERTRSLVEDLMGKRPELRFKFIQDNAKFVKADDI
DV