Protein Info for MPMX19_00948 in Azospirillum sp. SherDot2

Annotation: Protein-glutamate methylesterase/protein-glutamine glutaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 PF00072: Response_reg" amino acids 9 to 111 (103 residues), 75.1 bits, see alignment E=5e-25 PF01339: CheB_methylest" amino acids 175 to 352 (178 residues), 163.6 bits, see alignment E=3.8e-52

Best Hits

Swiss-Prot: 40% identical to CHEB2_DESVH: Protein-glutamate methylesterase/protein-glutamine glutaminase 2 (cheB2) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: None (inferred from 87% identity to azl:AZL_016650)

Predicted SEED Role

"Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)" in subsystem Bacterial Chemotaxis (EC 3.1.1.61)

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.61

Use Curated BLAST to search for 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (359 amino acids)

>MPMX19_00948 Protein-glutamate methylesterase/protein-glutamine glutaminase (Azospirillum sp. SherDot2)
MTDPRIRLLVVDDSNFVRIALRKMLEPAPDIEIVGEARNGAEALRLSRKLKPHVVAMDLN
MPVMDGIESVQAIAAEKGPPCIMVSNETSEGAAATIRALETGAVDFVCKNSSFITFDMAS
IERQLTEKVRHWGRWRMALTGERAADSVPAPVADPAPEPPPPLRPSVRGHRPDLVVVGVS
TGGPVALAELLGALGPLPCPLVVAQHMPASFTAGFAASLARSLGRPVAEGADGETLDPTA
VTILRGGGDWTVRRGTSGRFILHAADPAGAIYHPSADRLFRSAAEAAESPVAVVLTGMGD
DGSSGAAAFAERRFPVLVQDPATCAVDGMPRAAIRAGIATEVLPVTSIARKLNALFTLS