Protein Info for MPMX19_00868 in Azospirillum sp. SherDot2

Annotation: Sensor kinase CckA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 860 transmembrane" amino acids 35 to 57 (23 residues), see Phobius details amino acids 63 to 81 (19 residues), see Phobius details PF13188: PAS_8" amino acids 99 to 154 (56 residues), 24.1 bits, see alignment 8.8e-09 amino acids 220 to 257 (38 residues), 14.7 bits, see alignment (E = 8.5e-06) amino acids 344 to 390 (47 residues), 32 bits, see alignment 2.9e-11 PF13426: PAS_9" amino acids 229 to 330 (102 residues), 14 bits, see alignment E=1.8e-05 amino acids 355 to 407 (53 residues), 21.5 bits, see alignment 8.2e-08 TIGR00229: PAS domain S-box protein" amino acids 340 to 437 (98 residues), 39.9 bits, see alignment E=2.1e-14 PF00989: PAS" amino acids 345 to 409 (65 residues), 28.1 bits, see alignment 6.4e-10 PF08448: PAS_4" amino acids 350 to 440 (91 residues), 33 bits, see alignment E=2.2e-11 PF00512: HisKA" amino acids 489 to 551 (63 residues), 37.1 bits, see alignment 9e-13 PF02518: HATPase_c" amino acids 595 to 715 (121 residues), 81.2 bits, see alignment E=2.8e-26 PF00072: Response_reg" amino acids 742 to 852 (111 residues), 74.6 bits, see alignment E=2.4e-24

Best Hits

KEGG orthology group: K13587, two-component system, cell cycle sensor histidine kinase and response regulator CckA [EC: 2.7.13.3] (inferred from 91% identity to azl:AZL_012120)

Predicted SEED Role

"sensory box histidine kinase/response regulator"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (860 amino acids)

>MPMX19_00868 Sensor kinase CckA (Azospirillum sp. SherDot2)
MSRPDRVGFGVLVDDTTSLSDSARNAVNRKASSGGVLMLVQYALAPAGLLVLAAGLLLER
DAVGWLGAALVALGVVLLIARSVSVVRRSAHIGALLGGALEGMPAGQLVCDGTDDVVFVN
AAFRELAGWRRGERPLDALARQFSGDPDSAREFRRLRERVRAGGAASAELAIPALDGHPP
EWRKVQGQPVAGFPGSVHWRIEDITARRELEHVMLREQAKLADFMEHAPVGFFSVDQNGH
FLFVNATLAQWLEAAPGDLTDGEARLHGILAAPPVSAAPYDLFDHGGGEQRGELTMVGLG
RRRFQVAVAQSVVHADDGTIHTRSVVRDLSPEREWQEALRQSEQRFQRFFEDAPIGIALV
DETGQLTECNEAFLALIGSETGSVINRPMADLIVPAEREAVIARLRAVQGGADPAHPLEV
RLSGGRELTAQLYARRLANPVRTDGRADGARGDGARGDGVGLILHFIDMTERKSLEAQFA
QSQKMQAVGQLAGGVAHDFNNLLTAMIGFCDLLLLRHKPGDQSFSDIMQIKQNANRAANL
VRQLLAFSRQQTLQPRVINVTDVLAELANLLRRLIGENIELKMIHGRDLGLVKVDQNQLE
QVIINLVVNARDAMAGGGKLIVNTTNYVVSQPERREHETVPAGSYVSIEVIDTGCGIARE
NLQRIFEPFYTTKEVGSGTGLGLSTVYGIVRQTGGFVLVDSAVGEGTTFTILLPHHQGEL
RPVDTAEPRERRGSDLTGTGIILLVEDEDAVRVFSARALRNKGYQVLEAKNGEAALQQIE
TNGASIDLLITDVVMPQMDGPTLARHVRRLRPEMRVIFISGYAEDRLGEIDGVEVAHFLP
KPFSLKQLASKVKEVIRDGR