Protein Info for MPMX19_00838 in Azospirillum sp. SherDot2

Annotation: GTP pyrophosphokinase rsh

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 719 TIGR00691: RelA/SpoT family protein" amino acids 26 to 713 (688 residues), 718 bits, see alignment E=5.8e-220 PF13328: HD_4" amino acids 26 to 176 (151 residues), 162.5 bits, see alignment E=2e-51 PF01966: HD" amino acids 46 to 144 (99 residues), 45.7 bits, see alignment E=2.2e-15 PF04607: RelA_SpoT" amino acids 237 to 347 (111 residues), 139.5 bits, see alignment E=1.6e-44 PF02824: TGS" amino acids 389 to 447 (59 residues), 74.1 bits, see alignment 2.2e-24 PF19296: RelA_AH_RIS" amino acids 458 to 626 (169 residues), 132.3 bits, see alignment E=6.6e-42 PF13291: ACT_4" amino acids 641 to 715 (75 residues), 54 bits, see alignment E=6.7e-18

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 98% identity to azl:AZL_011780)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (719 amino acids)

>MPMX19_00838 GTP pyrophosphokinase rsh (Azospirillum sp. SherDot2)
MIRQYELVERVKAYDPSADEDLLNRAYVFSMKAHGSQTRASGDPYFLHPLEVAGILTQMK
LDAGTIATALLHDTVEDTVATLEDIERVFGKEIARLVDGVTKLSRLELNSEQAKQAENFR
KLVIAMSEDIRVLLVKLADRLHNMRTLFHLKKPEKRRRIARETIEIYSPLAERIGMHKIK
DELDDLAFAELNPDARDSILAQLARLRSEGENRVQTIITELRELLASEGLPDVSVSGREK
SAYSIWRKLQRKNVSFEQLSDIMAFRITVGSVGECYQALGIVHAHYPVVPGRFKDYISTP
KPNGYRSLHTGVIGPGRNRIEVQIRTQDMHEIAELGVAAHWAYKQDHQPRPNGGEYRWLR
ELLDILEHAQKPEEFLEHTKLELFQDQVFCFTPKGDLIALPRGATPVDFAYAVHSQVGDH
CVGAKINGRMLPLRTQLQNGDQVDIVTSKAQTPVPGWERFVVTGKARARIRKFLRTQQRA
QYMELGRGMLMRQFKSEGYEFTEKALEAATKIFQQPTVDDLMAGVGSGLHSVREVFHAVF
PGHKAQAAPAVRELEESAPKPKAKARKESALPIRGLIPGMAVHYARCCHPLPGDRIVGIV
TTGKGVTIHTIDCETLESFHESPERWIDVSWETGPDSPEEHVGRVSVVIANEQGSLGTLF
TVIGKNQGNVIHQKITNRSTDYFELLIDIDVKDAKHLTNIMAALRATPAIHSVERARGR