Protein Info for MPMX19_00751 in Azospirillum sp. SherDot2

Annotation: 50S ribosomal protein L3 glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 14 to 303 (290 residues), 389.9 bits, see alignment E=6.7e-121 TIGR00536: methyltransferase, HemK family" amino acids 16 to 304 (289 residues), 214.5 bits, see alignment E=1.6e-67 PF03602: Cons_hypoth95" amino acids 137 to 223 (87 residues), 26.2 bits, see alignment E=3.5e-09 PF05175: MTS" amino acids 141 to 223 (83 residues), 62.2 bits, see alignment E=3e-20 PF09445: Methyltransf_15" amino acids 142 to 217 (76 residues), 27.5 bits, see alignment E=1.3e-09 PF06325: PrmA" amino acids 142 to 215 (74 residues), 38.7 bits, see alignment E=5e-13 PF13847: Methyltransf_31" amino acids 142 to 268 (127 residues), 52.9 bits, see alignment E=2.2e-17 PF13649: Methyltransf_25" amino acids 144 to 221 (78 residues), 45.1 bits, see alignment E=8e-15 PF08242: Methyltransf_12" amino acids 145 to 215 (71 residues), 31.3 bits, see alignment E=1.7e-10 PF08241: Methyltransf_11" amino acids 145 to 214 (70 residues), 27.5 bits, see alignment E=2.4e-09 PF01170: UPF0020" amino acids 160 to 219 (60 residues), 29.7 bits, see alignment E=3.1e-10

Best Hits

Swiss-Prot: 60% identical to PRMB_BRADU: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 95% identity to azl:AZL_010480)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>MPMX19_00751 50S ribosomal protein L3 glutamine methyltransferase (Azospirillum sp. SherDot2)
MTSKHTAASAAAELSTIRDFLRYGVSRFNEADLDYGHGTTTAFDEAVFLVLESLHLPVDQ
LDPYLDSRLTAAEREAVAGILHARIDTRKPAPYLLNKAYIQGIPFYVDERVIVPRSYIGE
LLFSDMFGGDDFTLVEDPTSVERVLDLCTGSGCLAILAARIFPEAQVDAVDLSPDALEVA
KRNVADSGFADRITLHQGDLFAPLKTRKYDVIITNPPYVDAEAMDALPPEFRAEPALALA
GGDDGLDIVRRILKEAPKHLTPEGGLLCEFGTGREILEAEYPQLDFLWLQTANSFGEVFW
LTRDQLKAGK