Protein Info for MPMX19_00729 in Azospirillum sp. SherDot2

Annotation: K(+)-insensitive pyrophosphate-energized proton pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 55 to 74 (20 residues), see Phobius details amino acids 77 to 96 (20 residues), see Phobius details amino acids 126 to 151 (26 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 237 to 257 (21 residues), see Phobius details amino acids 262 to 283 (22 residues), see Phobius details amino acids 295 to 320 (26 residues), see Phobius details amino acids 332 to 355 (24 residues), see Phobius details amino acids 382 to 405 (24 residues), see Phobius details amino acids 412 to 432 (21 residues), see Phobius details amino acids 475 to 493 (19 residues), see Phobius details amino acids 513 to 536 (24 residues), see Phobius details amino acids 581 to 602 (22 residues), see Phobius details amino acids 609 to 631 (23 residues), see Phobius details amino acids 679 to 699 (21 residues), see Phobius details TIGR01104: V-type H(+)-translocating pyrophosphatase" amino acids 6 to 699 (694 residues), 884.3 bits, see alignment E=3.7e-270 PF03030: H_PPase" amino acids 13 to 692 (680 residues), 868.9 bits, see alignment E=1.4e-265

Best Hits

Swiss-Prot: 69% identical to HPPA_BRADU: K(+)-insensitive pyrophosphate-energized proton pump (hppA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01507, inorganic pyrophosphatase [EC: 3.6.1.1] (inferred from 96% identity to azl:AZL_010110)

Predicted SEED Role

"Pyrophosphate-energized proton pump (EC 3.6.1.1)" in subsystem Phosphate metabolism (EC 3.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.1

Use Curated BLAST to search for 3.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>MPMX19_00729 K(+)-insensitive pyrophosphate-energized proton pump (Azospirillum sp. SherDot2)
MALAFIVIIAAGLLALAYGFRTGKEVMAASPGSERMQAIAAAVQEGARAYLNRQYTTIAI
AGAVLLVILWVTLGFPVALGFLIGAVLSGSAGYVGMNVSVRANVRTAEAATRGLAPALDI
AFKSGAITGMLVVGLGLLGVGGYFGILTLVYRVNDPVEVRTVLEALVALSFGASLISIFA
RLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAGMAADLFETYAV
TIVATMLLAAIFFTGGVLRMMLVYPLLIGAVCILASVAGTFAVKLGPDNNIMKALYKGLA
VAAGISLVLILIVTAILFGFTRAITMTDGVSITGVNLLVSALVGLGVTGLLVWITEFYTS
TAFRPVRSVAKASETGHGTNVIQGLAVSMESTALPVIVICAGILIAHGQAGVFGIGIAAT
TMLALAGMVVALDAYGPVTDNAGGIAEMSDMPASIRVTTDALDAVGNTTKAVTKGYAIGS
AGLAALVLFAAYVQDLGHYFPNISVEFRLNDPYVVVGLLVGGLLPYLFGAMGMTAVGRAA
GSVVVEVRRQFREIPGIMEGTAKPDYGRAVDMLTKAAIKEMIIPSLLPVLAPVVLYLVIA
AIGGRAAGFASLGAMLLGTIVTGIFVAISMTSGGGAWDNAKKFIEEGNHGGKGSDAHKAA
VTGDTVGDPYKDTAGPAVNPMIKITNIVAILLLAILAQVG