Protein Info for MPMX19_00716 in Azospirillum sp. SherDot2

Annotation: Hydrogenase maturation factor HypD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 TIGR00075: hydrogenase expression/formation protein HypD" amino acids 2 to 368 (367 residues), 457.7 bits, see alignment E=1.3e-141 PF01924: HypD" amino acids 13 to 365 (353 residues), 503.5 bits, see alignment E=1.6e-155

Best Hits

Swiss-Prot: 68% identical to HYPD_RHILV: Hydrogenase maturation factor HypD (hypD) from Rhizobium leguminosarum bv. viciae

KEGG orthology group: K04654, hydrogenase expression/formation protein HypD (inferred from 96% identity to azl:AZL_009980)

MetaCyc: 48% identical to Fe-(CN)2CO cofactor assembly scaffold protein HypD (Escherichia coli K-12 substr. MG1655)
RXN-22646; RXN-22647

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypD" in subsystem NiFe hydrogenase maturation

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>MPMX19_00716 Hydrogenase maturation factor HypD (Azospirillum sp. SherDot2)
MKYVEEFRDPVLARNVAAAIAREVRADRSYHLMEFCGGHTHAISRYGIPDLLPGNVRMIH
GPGCPVCVLPVGRIDDAIALARRPEVTLCTYGDVMRVPGSGRLSLLKAKAQGADVRMVVT
ADAAVRIAAENPDRQVVFLAIGFETTTPPTALAVRAAAAQGLTNFSVFCNHVLTPSAIQG
ILAGADEGLSLDGFVGPAHVSVVIGSEAYAPAAAEHGKPVVISGFEPLDVLQSILMLVRQ
INDGRAEVENQFTRAVTADGNRKAQALVLEIFETRPSFEWRGLGSIPHSGLKLRDAYAAF
DAERRFPIAGASVADHKGCDCGAILRGVKRPVDCKLFDTVCTPENPMGSCMVSAEGACAA
HYTYGRFRDA