Protein Info for MPMX19_00698 in Azospirillum sp. SherDot2

Annotation: Holliday junction ATP-dependent DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 PF05496: RuvB_N" amino acids 24 to 182 (159 residues), 265.9 bits, see alignment E=3.8e-83 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 28 to 330 (303 residues), 448 bits, see alignment E=7.2e-139 PF06068: TIP49" amino acids 31 to 85 (55 residues), 27.4 bits, see alignment 6.7e-10 PF07728: AAA_5" amino acids 58 to 164 (107 residues), 25.8 bits, see alignment E=3.3e-09 PF00004: AAA" amino acids 59 to 181 (123 residues), 74.8 bits, see alignment E=3.2e-24 PF17864: AAA_lid_4" amino acids 185 to 258 (74 residues), 99.7 bits, see alignment E=2.2e-32 PF05491: RuvB_C" amino acids 260 to 329 (70 residues), 88 bits, see alignment E=1.1e-28

Best Hits

Swiss-Prot: 81% identical to RUVB_RHOCS: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 97% identity to azl:AZL_009790)

MetaCyc: 61% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (357 amino acids)

>MPMX19_00698 Holliday junction ATP-dependent DNA helicase RuvB (Azospirillum sp. SherDot2)
MSDPNHDRLVQPGQGHGDSAEASIRPLSLAEFIGQRQARENLSIFIQAARSRNEALDHVL
LFGPPGLGKTTLAQIVARELGVGFRATSGPVIARAGDLAALLTNLQPHDVLFIDEIHRLN
PAVEEVLYPAMEDFQLDLIIGEGPSARSIRIDLPPFTLVGATTRSGLITRPLRERFGIPV
RLQFYEPDELELIVRRAAGVLGMGITPEGAREIANRARGTPRVSGRLLRRVRDFAAVAGV
EEVDKRVADAALTRLEVDRLGLDSMDRRYLGLVANNYGGGPVGVETLGAALGEQRDVLEE
VVEPYLIQQGFLQRTPRGRMLTDQGFRYLGLNPPNAPLRQLDLLDRVPDPTDGDGDE