Protein Info for MPMX19_00667 in Azospirillum sp. SherDot2

Annotation: Single-stranded-DNA-specific exonuclease RecJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 598 transmembrane" amino acids 204 to 225 (22 residues), see Phobius details amino acids 245 to 264 (20 residues), see Phobius details TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 40 to 591 (552 residues), 525.3 bits, see alignment E=7.5e-162 PF01368: DHH" amino acids 94 to 250 (157 residues), 75.1 bits, see alignment E=9.2e-25 PF02272: DHHA1" amino acids 337 to 465 (129 residues), 70.9 bits, see alignment E=2.3e-23 PF17768: RecJ_OB" amino acids 483 to 591 (109 residues), 74.8 bits, see alignment E=8.3e-25

Best Hits

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 96% identity to azl:AZL_009490)

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (598 amino acids)

>MPMX19_00667 Single-stranded-DNA-specific exonuclease RecJ (Azospirillum sp. SherDot2)
MTEPAAFLNVANSLSGKRWQARPCDERQAQALTQGRGLPEIVGRVLAARGVTEDSCETFL
NPTLKALLPDPSRFRDMDPAAERIARAIRDGEPVAVFGDYDVDGATSAALLRRFFRSLGA
DIRVYVPDRITEGYGPNAPALLRLKGEGVRLVVTVDCGISAFAALEAAAEAGLDVVVLDH
HAAEPRLPPAVALVNPNRLDEDGAYRTLCAAGVTFITIVAINRVLRTAGFYRDRAEPNLM
EWLDIVALGTVCDVVPLTGLNRALVAQGLKVMARRANPGLAALSDVAGVKEKPDAYHAGY
VLGPRVNAGGRVGASDLGARLLSTDDPIEAMELAQRLEGHNAERRTIEQAVLDEALERVA
AEAGRTTDLVFVTGLGWHPGVIGIVAARLKERYSRPACVVALEETADGTVIGKASGRSVR
GVDLGAAVIAARQEGLLLAGGGHRMAAGFTVAGDRIDALRDFLHSRISEQVASAPLVPTL
ELDGALSVGGASVGLVTTLDKLGPYGTGNAEPRFAIADARVVRADVVGANHVRCILQGND
GARLKAIAFRALDSDMGQALLTGRGTPFHLAGVLRIDRWNGSEGVQLLIDDAAPARSA