Protein Info for MPMX19_00647 in Azospirillum sp. SherDot2

Annotation: Bifunctional enzyme CysN/CysC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 6 to 403 (398 residues), 343.7 bits, E=2.3e-106
• PF00009: GTP_EFTU
  • amino acids 6 to 188 (183 residues), 161.2 bits, E=7.8e-51
• PF22594: GTP-eEF1A_C
  • amino acids 313 to 405 (93 residues), 48 bits, E=4.9e-16
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 424 to 603 (180 residues), 235.1 bits, E=6.8e-74
• PF01583: APS_kinase
  • amino acids 438 to 586 (149 residues), 212.7 bits, E=9.5e-67

Best Hits

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 96% identity to azl:AZL_009320)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (4/12 steps found)
• superpathway of seleno-compound metabolism (7/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.25, 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Protein Sequence (622 amino acids)

>MPMX19_00647 Bifunctional enzyme CysN/CysC (Azospirillum sp. SherDot2)
MPHSQLRIVIVGHVDHGKSTLIGRLLNDTGSLPDGKVEQVREMSRKRGMPFEWAFVMDAL
QAERDQGITIDTTQIHFKTKQRPYVIIDAPGHTEFLKNMVTGASQADAALLVIDADEGAL
EQSRRHAFLLHLLGVRQVAVVVNKMDKVEFSSHRYEVVAQEIKDYLAELGLQPKAVIPVS
ARNGDNIVSRSEQSDWYLGPTVIEQLDAFRPQQTSPDQPLRLPLQDVYKPDDRRILAGRI
ESGRLRVGDTLHFSPTGASAKVVSIEAWNHPETVLSAQAGQSIGITLDKRIFAERGHIAH
HQSDAPTLAHRLSVRLFWLVSDPLVVGKTYTLKIATGEHRVTVEQVTAVINVEDLSSTPG
KAVRRNEVAEVVLRSRSPIALDLASDLPRTGRGALIDGNDVVGGCLVVEIEDAAAANITE
VSHTVSLEERAQSNGHKGGVIWLTGLSGAGKSTLAMALERALFDRGWQTFVLDGDNVRNG
LNAGLGFSSDDRAENIRRVAETAKLFADAGMVVIASLISPLKADRARARVIGGERFHEVH
VAANLATCEERDPKGLYKKARAGEIAEFTGISAPYEAPDQPELVIDTDALSRSEALAQLL
DYVDETLGKNSLKSEKELTYVI