Protein Info for MPMX19_00619 in Azospirillum sp. SherDot2

Annotation: Diacetyl reductase [(S)-acetoin forming]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 7 to 196 (190 residues), 198.3 bits, E=2.3e-62
• PF08659: KR
  • amino acids 9 to 168 (160 residues), 53.9 bits, E=5.6e-18
• PF01370: Epimerase
  • amino acids 9 to 180 (172 residues), 23.4 bits, E=8.9e-09
• PF13561: adh_short_C2
  • amino acids 13 to 258 (246 residues), 204.7 bits, E=4.3e-64

Best Hits

Swiss-Prot: 34% identical to STCU_EMENI: Versicolorin reductase (stcU) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

KEGG orthology group: K03366, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 95% identity to azl:AZL_009050)

MetaCyc: 39% identical to galactitol 2-dehydrogenase (Agrobacterium fabrum C58)
Galactitol 2-dehydrogenase. [EC: 1.1.1.16]

Predicted SEED Role

"2,3-butanediol dehydrogenase, S-alcohol forming, (S)-acetoin-specific (EC 1.1.1.76)" in subsystem Acetoin, butanediol metabolism (EC 1.1.1.76)

MetaCyc Pathways

• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• superpathway of (R,R)-butanediol biosynthesis (4/5 steps found)
• (R,R)-butanediol biosynthesis (1/1 steps found)
• (S,S)-butanediol biosynthesis (1/1 steps found)
• (R,R)-butanediol degradation (1/1 steps found)
• (S,S)-butanediol degradation (1/1 steps found)
• superpathway of 2,3-butanediol biosynthesis (3/6 steps found)
• D-altritol and galactitol degradation (1/4 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Galactose metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.4

Use Curated BLAST to search for 1.1.1.16 or 1.1.1.303 or 1.1.1.4 or 1.1.1.76

Protein Sequence (261 amino acids)

>MPMX19_00619 Diacetyl reductase [(S)-acetoin forming] (Azospirillum sp. SherDot2)
MPDLKDKCIIVTGAGRGIGATIARALAADGAKLTIADRNAADAQQVAEAIRAAGGSAIAV
TVDVRDRTAVRRMIDETVKAHGRLDVLFNNAGIAQTKPFLDITEDDWHTVNDVNALGVLI
GMQEAIKTFRKQGGGGKIVNTASIAGKQGYEPLAHYSASKFAVVALTQAAARGFGKDGIT
ANAICPGVVATEMWKIIDQGFRDTGITKSENEAFDMFAAGAVLGRPSRPDDLVGVARFLA
SSDSDFMTGQSLLVDGGMVFA