Protein Info for MPMX19_00611 in Azospirillum sp. SherDot2
Annotation: (3S)-malyl-CoA thioesterase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 38% identical to CITE_ECOL6: Citrate lyase subunit beta (citE) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 92% identity to azl:AZL_008940)MetaCyc: 38% identical to citrate lyase beta subunit (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"L-malyl-CoA/beta-methylmalyl-CoA lyase (EC 4.1.3.-)" (EC 4.1.3.-)
MetaCyc Pathways
- citrate degradation (2/2 steps found)
- reductive TCA cycle II (7/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- Citrate cycle (TCA cycle)
- Pyruvate metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.-, 4.1.3.34, 4.1.3.6
Use Curated BLAST to search for 4.1.3.- or 4.1.3.34 or 4.1.3.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (289 amino acids)
>MPMX19_00611 (3S)-malyl-CoA thioesterase (Azospirillum sp. SherDot2) MATTIRPRRSVLYMPGSNARALEKGRSLPADGLILDLEDAVAPDAKAAARDTIAGALAAG GYGGRELIVRVNGLNTPWGYDDLRMAAASGADAVLLPKVESADAVRQAEAVLRAAGAPAG QTIWCMMETPLGILNAKEIAGASPAVGGLVLGTSDLAKDLHAAHTAQRLPMITSLGLCLL AARAYGLAVLDGVHLDLNDEDGFAASCRQGRELGFDGKTLIHPKTIAACNAAFAPDADEI AQAHRIIAAHAEAAAAGKGVVLVDGKLVENLHVENARRLVTLAEAIDAL