Protein Info for MPMX19_00599 in Azospirillum sp. SherDot2

Annotation: Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 PF00919: UPF0004" amino acids 14 to 98 (85 residues), 71.3 bits, see alignment E=5.6e-24 TIGR01579: MiaB-like tRNA modifying enzyme" amino acids 14 to 384 (371 residues), 443.9 bits, see alignment E=7.3e-137 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 14 to 383 (370 residues), 349.5 bits, see alignment E=2.6e-108 PF04055: Radical_SAM" amino acids 144 to 316 (173 residues), 82.6 bits, see alignment E=3.9e-27

Best Hits

KEGG orthology group: None (inferred from 97% identity to azl:AZL_008840)

Predicted SEED Role

"tRNA-t(6)A37 methylthiotransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>MPMX19_00599 Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB (Azospirillum sp. SherDot2)
MPDDVVLKTDGPEIVTFGCRLNTYESEVMRNHVRSAGLDDVVIVNTCAVTSEAERQARQT
IRKLRRERPDARIVVTGCAAQIDPQRFAAMPEVDQVLGNQEKLQPESWGLPPAEKVLVND
IMSVKETAGHLIGGFEDRARAFVQVQQGCDHRCTFCIIPYGRGPSRSVPIGGIVEQVQAL
VKAGYNEVVLSGVDITSYGPDLPGSPSLGQMVRRLLALVPELPRLRLSSIDCIEMDDDLW
RLIETEPRLMPHLHLSLQAGDDMVLKRMKRRHGRADSIAFCERVRSVRPDVVFGADFIAG
FPTETEEMFENTMRLVEECGLTWLHVFPYSPRPGTPAARMPQVDGAVRKERAARLRAVGE
AAEARTLAGLVGRTATVLVEKDDLGRTEHFAAIRLGRIFPPGSLVPAAITGIKDGVLTGT
PL