Protein Info for MPMX19_00588 in Azospirillum sp. SherDot2

Annotation: Nitrogenase iron protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 PF00142: Fer4_NifH" amino acids 7 to 278 (272 residues), 436.6 bits, see alignment E=8e-135 TIGR01287: nitrogenase iron protein" amino acids 7 to 281 (275 residues), 458.2 bits, see alignment E=4.6e-142 PF02374: ArsA_ATPase" amino acids 7 to 50 (44 residues), 26.6 bits, see alignment 6.9e-10 PF13614: AAA_31" amino acids 9 to 78 (70 residues), 35.7 bits, see alignment E=1.7e-12 PF01656: CbiA" amino acids 11 to 230 (220 residues), 34.9 bits, see alignment E=2.7e-12

Best Hits

Swiss-Prot: 95% identical to NIFH_AZOBR: Nitrogenase iron protein (nifH) from Azospirillum brasilense

KEGG orthology group: K02588, nitrogenase iron protein NifH [EC: 1.18.6.1] (inferred from 100% identity to azl:AZL_007710)

MetaCyc: 74% identical to [FeMo]-nitrogenase complex reductase component monomer (Trichormus variabilis)

Predicted SEED Role

"Nitrogenase (molybdenum-iron) reductase and maturation protein NifH" in subsystem Nitrogen fixation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (298 amino acids)

>MPMX19_00588 Nitrogenase iron protein (Azospirillum sp. SherDot2)
MAKAPLRQIAFYGKGGIGKSTTSQNTLAALVELDQKILIVGCDPKADSTRLILHAKAQDT
VLHLAAEAGSVEDLELEDVLKIGYKNIKCVESGGPEPGVGCAGRGVITSINFLEENGAYD
DVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHSGGV
RLGGLICNERQTDKEWDLADALAKRLGSKLIHFVPRDNIVQHAELRRMTVIEYAPDSKQA
GEYRALANKIHANSGQGCIPTPITMEELEEMLMDFGIMKTEEQQLAELAAKEAAKAGA