Protein Info for MPMX19_00587 in Azospirillum sp. SherDot2

Annotation: Nitrogenase molybdenum-iron protein alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 TIGR01282: nitrogenase molybdenum-iron protein alpha chain" amino acids 14 to 479 (466 residues), 886 bits, see alignment E=5.4e-271 TIGR01862: nitrogenase component I, alpha chain" amino acids 30 to 472 (443 residues), 625.8 bits, see alignment E=3.5e-192 PF00148: Oxidored_nitro" amino acids 62 to 466 (405 residues), 339.7 bits, see alignment E=1.1e-105

Best Hits

Swiss-Prot: 89% identical to NIFD_AZOBR: Nitrogenase molybdenum-iron protein alpha chain (nifD) from Azospirillum brasilense

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 98% identity to azl:AZL_007700)

MetaCyc: 71% identical to [FeMo]-nitrogenase complex dinitrogenase component alpha subunit (Azotobacter vinelandii)
Nitrogenase (flavodoxin). [EC: 1.19.6.1]

Predicted SEED Role

"Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1 or 1.19.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (479 amino acids)

>MPMX19_00587 Nitrogenase molybdenum-iron protein alpha chain (Azospirillum sp. SherDot2)
MSLSENTTVDVKNLVNEVLEAYPEKSRKRRAKHLNVLEAEAKDCGVKSNVKSIPGVMTIR
GCAYAGSKGVVWGPIKDMIHISHGPVGCGYYSWSGRRNYYIGDTGVDSWGTMHFTSDFQE
KDIVFGGDKKLHKVIEEINELFPLVNGISIQSECPIGLIGDDIEAVARAKSAEIGKPVIP
VRCEGFRGVSQSLGHHIANDAIRDWVFEKTEPKAGFVSTPYDVTIIGDYNIGGDAWSSRI
LLEEIGLRVIAQWSGDGTLAELENTPKAKVNLIHCYRSMNYIARHMEEKFNIPWMEYNFF
GPSQIAESLRKIAALFDDKIKENAEKVIARYQPMVDAVIAKYKPRLEGKKVMIYVGGLRP
RHVVDAYHDLGMEITGTGYEFAHNDDYQRTQHYVKEGTLIYDDVTAFELEKFVEAMRPDL
VASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVWGVMKAPF