Protein Info for MPMX19_00548 in Azospirillum sp. SherDot2

Annotation: Periplasmic nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 966 PF04879: Molybdop_Fe4S4" amino acids 75 to 127 (53 residues), 39 bits, see alignment 9.4e-14 PF00384: Molybdopterin" amino acids 131 to 572 (442 residues), 151.2 bits, see alignment E=5.9e-48 PF01568: Molydop_binding" amino acids 833 to 959 (127 residues), 75.9 bits, see alignment E=3.8e-25

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 97% identity to azl:AZL_007310)

Predicted SEED Role

"Formate dehydrogenase-O, major subunit (EC 1.2.1.2)" in subsystem Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (966 amino acids)

>MPMX19_00548 Periplasmic nitrate reductase (Azospirillum sp. SherDot2)
MLIKKSSATSGGRHLARTLTQTLATATGVTVDRRAFLRTSGIAAGGAALASAMPFGMVKK
ADAATAAAVAGAPVKQVKSVCTHCSVGCTVVAEVQNGVWIGQEPGFDSPINLGSHCAKGA
SVREHAHGERRLRYPMKMVDGKWTRIGWDQAIEEVGSKLLSIREQSGPDSVFWLGSAKHS
NEQAYLFRKFAALWGTNNVDHQARICHSTTVAGVANVWGYGAMTNSYNDIQKSKALFFIG
SNAAEAHPVAMMHVLKAKEQNAAPLIVADPRFTRTAAHANEYIRFRPGTDVALVWGILWH
VLQNGWEDKDYIAKRVYGFDDIRAEVAKWTPEEVERVTGVPGSQLHRVARTLASNRPGTL
VWCMGGTQHHIGNNNTRAYCVLQLALGNVGVSGGGTNIFRGHDNVQGATDFGVTSDSLPG
YYGLAEGAWRHWARVWDLPYDGILKRFGSKALMESTGIPVSRWFDGVLEAKENLDQPNPV
KGMVFWGHAPNSQVRLPDMKKAMEKLELLVVVDPYPTMTAVLPDRKDNFYLLPAATQFET
SGSATASNRSVQWRDKIMEPLFESKTDHEIMYLFAKKFGFAEDLVKNVKVNGNEPSIEDI
TREWNRGMWSVGYTGQSPERLKLHMQHQGTFDKTTLRAVGGPCDGDYFGLPWPCWGTPEM
KHPGSANLYDTSLPVAEGGGAFRARFGVERNGVTLLAEGSYPEGSEIKDGYPEVTMAMLQ
KLGFDADLTPEELAVIQKIGGDKIGAVSWTTDLSGGIQRVAIKHGLNPPGNAKARCVAWN
FPDPVPIHREPLYTPRRDLVASYPTYKDTKSWRVPTLYKSIQDRDVSKEYPIILTSGRLV
EYEGGGDETRSNPWLAELQQDMFVEINPFDANNAGVKDGQLVWVEGPEKGKVKVKAMVTE
RVGRGVAFMPFHFGGVYQGQDLRSKYPAGADPIVLGEAANTATTYGYDSVTQMQETKTTL
CRIQAA