Protein Info for MPMX19_00525 in Azospirillum sp. SherDot2

Annotation: Isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 947 TIGR00392: isoleucine--tRNA ligase" amino acids 16 to 861 (846 residues), 879.5 bits, see alignment E=1.4e-268 PF00133: tRNA-synt_1" amino acids 29 to 657 (629 residues), 472 bits, see alignment E=4.6e-145 PF09334: tRNA-synt_1g" amino acids 59 to 112 (54 residues), 43.4 bits, see alignment 6.2e-15 amino acids 602 to 656 (55 residues), 25.6 bits, see alignment 1.6e-09 PF08264: Anticodon_1" amino acids 701 to 858 (158 residues), 84.8 bits, see alignment E=1.7e-27 PF06827: zf-FPG_IleRS" amino acids 916 to 943 (28 residues), 27.7 bits, see alignment (E = 5.6e-10)

Best Hits

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 96% identity to azl:AZL_007060)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (947 amino acids)

>MPMX19_00525 Isoleucine--tRNA ligase (Azospirillum sp. SherDot2)
MTRDYKSTVFLPRTDFPMRGGLPTKEPELLKRWAEMDLFGKLRAGAKGRKKFVLHDGPPY
ANGNIHIGHAVNKILKDVIVRFRQMQGFDADYVPGWDCHGLPIEWKIEEKYRKAGKNKDD
VPLIQFRAECRQFAEEWVGIQAAEFRRLGVEGDWKQPYATMTFPAEAQIVREIHKFAVNG
GLYKGFKPVMWSVVEKTALADAEVEYHDHTSTTVFARFPVWGSSAPEVDEASIVIWTTTP
WTLPGNRAIAYGDEIEYATFKVTAVAEGSKAVVGERLVLATTLAESVLKETGITEWKQLH
RFPGSRLAGTYCRHPLNGKGYDFKVPLLAGDFVTTDAGSGFVHIAPGHGEDDFRLGQANN
IEVPQTVGEDGRYYAHVPLFAGLAVYTDQGKTGPANKAVLEAFEECGSLLASNRIKHSYP
HSWRSKAPLIFRTTPQWFISMETNDLRKVALQAISDTRWVPSQGENRIRSMIESRPDWCI
SRQRAWGVPIALFVRKSTGEILRDEAVFARIADIFQQEGADAWFARDPQDFLGNSYAAED
FEQVRDIVDVWFESGSSHAFVLEQRLEELAWPADLYLEGSDQHRGWFHSSLLESCGTRGR
APYNAVLTHGFVLDEQGRKMSKSLGNVVAPQEVCDQYGADILRLWIINSDYSEDLRIGKE
IIKTQADLYRRLRNTLRYLLGALADYTPAERIDAAEMPELERWVLHRLSELDTLVREKVE
AYDFHALFVALHNFCAVELSAFYFDVRKDSLYCDAVDSVRRRSVRTVMEHLFSNLTAWLA
PILCFTAEEAWLARPEGLTEGAGWTDESVHLRGFPEVPAEWRNDALASKWEAVRTVRRVV
TGALELERANKAIGSSLQADPTVFVDSATKALLADVDFQDVCITSAIEVTDAAAPEGAFT
LPDVPGIAVAPALADGEKCERCWKVLEEVGTVHDHPTLCIRCSDAVA