Protein Info for MPMX19_00462 in Azospirillum sp. SherDot2

Annotation: Caffeyl-CoA reductase-Etf complex subunit CarE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 PF01012: ETF" amino acids 24 to 205 (182 residues), 154.3 bits, see alignment E=3.2e-49 PF00766: ETF_alpha" amino acids 230 to 309 (80 residues), 118.1 bits, see alignment E=1.3e-38

Best Hits

Swiss-Prot: 63% identical to FIXB_AZOC5: Protein FixB (fixB) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K03522, electron transfer flavoprotein alpha subunit (inferred from 98% identity to azl:AZL_006480)

MetaCyc: 59% identical to quinone reductase (NADH,flavodoxin) complex electron transfer flavoprotein component beta subunit (Azotobacter vinelandii)

Predicted SEED Role

"Electron transfer flavoprotein, alpha subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (359 amino acids)

>MPMX19_00462 Caffeyl-CoA reductase-Etf complex subunit CarE (Azospirillum sp. SherDot2)
MSEPSKPQGRKFQLPEHLKVYQNVWVIVEQERGIVHSVSLELLGEARKLADKLGVEVGAV
VLGAESPDLNRICGDAIGYGADIVYKVTDPALADYRTDPYCRVMTDVVNTHKPEIVLLGA
TTLGRDLAGAIATTLATGLTADCTELDIYADNRSLAATRPTFGGTLLCTIQTLAYRPQMA
TVRPRVMAMPERDDARTGRVIEIQPDLREEDIVTKVLNFIADREQNEAQLAFADIIVSAG
KGLGKVENMKLVFDLAKVLGAEVGVTRPLVQAGWATNDRQVGQTGKTVRPKLYIAAGISG
AIQHRVGMEKSDLILAINTDPNATIFDFAHLGLVGDALKILPALTDAFGRRLSVNRMAG