Protein Info for MPMX19_00418 in Azospirillum sp. SherDot2

Annotation: Putative glutamate--cysteine ligase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 TIGR02050: carboxylate-amine ligase, YbdK family" amino acids 11 to 296 (286 residues), 307.9 bits, see alignment E=3.6e-96 PF04107: GCS2" amino acids 12 to 292 (281 residues), 193.5 bits, see alignment E=2.6e-61

Best Hits

Swiss-Prot: 65% identical to GCS2_RHORT: Putative glutamate--cysteine ligase 2 (Rru_A2462) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K06048, carboxylate-amine ligase [EC: 6.3.-.-] (inferred from 94% identity to azl:AZL_006070)

Predicted SEED Role

"Carboxylate-amine ligase bll3764 (EC 6.3.-.-)" (EC 6.3.-.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>MPMX19_00418 Putative glutamate--cysteine ligase 2 (Azospirillum sp. SherDot2)
MESTVTEPSFTLGLEEEYLLVDRGTRDIVPSPPDEMLEACQEQAPGRIHPEFLRCQIEVG
TPVCKTLDEARAELAALRGTVAGVADRYGLAPIAASTHPFAAWEPQKHTNRDRYNMLARD
LGAPARRLMICGMHVHVGIEDDDLRIDLMNQVRYFLPHLLALSTSSPFWRGRDMERRSYR
LSVWHELPRTGMPAGFQSYGEYQSHVAVLVNAGVIEDASKLWWDIRPSSRFPTLEMRITD
ICTRLDDAVTVAALYRCLLRMLWRLKLRNVTWRPYKNLLIEENRWRAQRYGLDSGLVDFG
QGRIVPYADLLDEIIDLTREDAEHFGCTAEVGRARDILRRGTSAHRQIEIFQAALADGAS
EDEALRRVVDWLAEETMVGVRDEPSPAGVTA