Protein Info for MPMX19_00123 in Azospirillum sp. SherDot2

Annotation: ATP-dependent DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 23 to 635 (613 residues), 794.8 bits, see alignment E=4.8e-243 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 24 to 473 (450 residues), 514.2 bits, see alignment E=3.3e-158 PF00270: DEAD" amino acids 37 to 196 (160 residues), 83.3 bits, see alignment E=4.2e-27 PF00271: Helicase_C" amino acids 237 to 340 (104 residues), 68.2 bits, see alignment E=1.8e-22 PF16124: RecQ_Zn_bind" amino acids 352 to 414 (63 residues), 74.1 bits, see alignment E=3.1e-24 PF09382: RQC" amino acids 416 to 524 (109 residues), 127.1 bits, see alignment E=7.3e-41 PF00570: HRDC" amino acids 567 to 633 (67 residues), 70 bits, see alignment E=3.4e-23

Best Hits

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 97% identity to azl:AZL_025580)

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (637 amino acids)

>MPMX19_00123 ATP-dependent DNA helicase RecQ (Azospirillum sp. SherDot2)
MFSYGNLGASDDAPGQPPATALEALRTVFGYDSFRGQQADIIDHVVRGGDALVLMPTGGG
KSLCYQIPALVRDGVTVVVSPLIALMRDQVTALRELGVRAAFLNSSLGPAEAREVERAMV
QGEIDLVYVAPERLVTPRFLDLLDRTRLALFALDEAHCVSQWGHDFRPEYLQLSILHERH
PMVPRIALTATADVQTRAEIKDKLGLTDARVFLSSFDRPNITYRVVPKKSERQQMLAFLR
DNHPEDAGIVYCMSRNKVEEVAAWLNQQGREALPYHAGLPAETREANQDRFIKSEGLVMV
ATVAFGMGIDKPNVRFVCHLDPPKSLEAYYQETGRAGRDGLPANAWMAYGMADVVMLRQM
LEQSEAGDSHRRVERGKLEALLGFCETSDCRRQVLLNYFNETLPEPCGNCDTCLDPVETW
DGTIAAQKALSAVYRTGQRYGAGHLIDVLVGNATEKVVQQNHDQLKTFAVGKDLTKVEWQ
SVYRQLVATGLLTVDLEGYGGFRLTDAGVAVIKGQRVVKLRKDPVLERRRGVHDALRRHT
ARGGSVGGSGEGVSRSGARGALSAADDALWHALKDCRTALAKAQSVPPYVIFHDSTLLEM
VAQRPRDHATFAHLPGVGGRKLERYADAFLEVIRQHG