Protein Info for MPMX19_00084 in Azospirillum sp. SherDot2

Annotation: Phenylalanine--tRNA ligase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 803 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 802 (802 residues), 712.8 bits, see alignment E=3e-218 PF01588: tRNA_bind" amino acids 45 to 145 (101 residues), 77.9 bits, see alignment E=1.3e-25 PF03483: B3_4" amino acids 217 to 389 (173 residues), 219.9 bits, see alignment E=4.6e-69 PF03484: B5" amino acids 409 to 475 (67 residues), 63.1 bits, see alignment E=5.7e-21 PF17759: tRNA_synthFbeta" amino acids 488 to 688 (201 residues), 145.8 bits, see alignment E=3.4e-46 PF03147: FDX-ACB" amino acids 709 to 802 (94 residues), 95.8 bits, see alignment E=4.3e-31

Best Hits

Swiss-Prot: 61% identical to SYFB_MAGSA: Phenylalanine--tRNA ligase beta subunit (pheT) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 95% identity to azl:AZL_025980)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (803 amino acids)

>MPMX19_00084 Phenylalanine--tRNA ligase beta subunit (Azospirillum sp. SherDot2)
MKFTLSWLKDHLETDASLDQITEKLTALGLEVEGIQDRSKELAPFRVAHVVSAEKHPDAD
KLRVLMVDTGAGTLQVVCGAPNARAGMKGVFAPEGTYVPGSDITLKKGVIRGVESNGMMC
SKRELKLSDEHEGIIELPDDAPVGAGFADYAGLNDPVIDINLTPDRADCAGIRGIARDLA
AAGMGTLKPLTEGHLNATPVDGRFASPVGVSIEVPDACPLFVGRYIRGVKNGPSPKWLQD
KLVSIGLRPISALVDITNYLTFDAARPLHVFDADTVKGNITVRMGREGETLAALNGKEYA
LDGTMTVVADDERAEALAGIVGGEPTGCTEQTVNVFVEAALFDPVRTAQTGRRLGIDSDA
RYRFERGVDPAAVFAGMERATRLILELCGGEPSELVVAGAEPAWKRSLSLRPGRVAALGG
VELPRERQERILTDLGFSLEGEDAEGRLVVGVPSWRADVHGEADLVEEVLRVHGFDAIPA
TPLPRETVLTRPALTLKQRRVALAKRTLAARGLSEAVTWSFLSGPVAGLFGGGQPGLRLV
NPISSDLDVMRPSVLPNLIQAAGRNADRGFADVRLFEVGAAFRTPAPDGQDTVAAGIRAG
AAVPRHWSEKARGVDVFDAKADALAVLEAVGAPAGNLQVTTDAPGWYHPGRSGVLRLGPT
VMARFGEIHPSVLDALGVKGPVVGFEVMLDAVPLPKKKGGTAKPMVQLSAFQPVERDFAF
VVDRKVEADKILRAVKGADKALVKDVAVFDVYEGPGVGEGRKSVAVSVTYQPTTATLTDE
AIEAVGQKIVAAVVKATGGNLRA