Protein Info for MPMX19_00083 in Azospirillum sp. SherDot2

Annotation: Phenylalanine--tRNA ligase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 PF02912: Phe_tRNA-synt_N" amino acids 18 to 85 (68 residues), 84.7 bits, see alignment E=3.4e-28 TIGR00468: phenylalanine--tRNA ligase, alpha subunit" amino acids 37 to 338 (302 residues), 341.4 bits, see alignment E=2.7e-106 PF01409: tRNA-synt_2d" amino acids 90 to 338 (249 residues), 342.4 bits, see alignment E=1.6e-106

Best Hits

Swiss-Prot: 76% identical to SYFA_RHOCS: Phenylalanine--tRNA ligase alpha subunit (pheS) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K01889, phenylalanyl-tRNA synthetase alpha chain [EC: 6.1.1.20] (inferred from 98% identity to azl:AZL_025990)

MetaCyc: 55% identical to phenylalanine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Phenylalanine--tRNA ligase. [EC: 6.1.1.20]; 6.1.1.20 [EC: 6.1.1.20]; 3.1.1.- [EC: 6.1.1.20]

Predicted SEED Role

"Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (355 amino acids)

>MPMX19_00083 Phenylalanine--tRNA ligase alpha subunit (Azospirillum sp. SherDot2)
MLDALKDELLSQVNAAADLSALDEVRVSALGKKGRITGFMKELGNLTPDERKERGQALNA
LKDEIAAAIDARKADLAAAHLKARLEAERVDVTLPVRPETEGRIHPISQTMDEMIAIFAE
MGFSVAEGPDIEDDFHNFTALNFPPGHPARDMHDTFYLPDADEKKMLLRTHTSPVQVRTM
LNNKPPIRIIAPGRTYRSDYDMTHTPMFHQIEGLVIDEATNMGHLKGCLVEFCRAFFDVD
DLPLRFRPSFFPFTEPSAEVDIGCSRKGGELKLGNYGDWLEILGCGMVHPNVLEACGIDS
TKYQGFAFGMGVERVAMLKYGIPDLRTFFEADLRWLKHYGFAALDIPNIAHGLTR