Protein Info for MPMX19_00056 in Azospirillum sp. SherDot2

Annotation: Leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 TIGR00396: leucine--tRNA ligase" amino acids 4 to 854 (851 residues), 1061.2 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 13 to 224 (212 residues), 95.5 bits, see alignment E=6.3e-31 amino acids 419 to 575 (157 residues), 30.9 bits, see alignment E=2.7e-11 amino acids 615 to 655 (41 residues), 34 bits, see alignment 2.9e-12 PF09334: tRNA-synt_1g" amino acids 36 to 170 (135 residues), 64.8 bits, see alignment E=1.3e-21 amino acids 615 to 658 (44 residues), 21.2 bits, see alignment 2.3e-08 PF13603: tRNA-synt_1_2" amino acids 220 to 404 (185 residues), 228.4 bits, see alignment E=1e-71 PF08264: Anticodon_1" amino acids 699 to 818 (120 residues), 73.8 bits, see alignment E=2.9e-24

Best Hits

Swiss-Prot: 74% identical to SYL_MAGSA: Leucine--tRNA ligase (leuS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 94% identity to azl:AZL_026240)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (854 amino acids)

>MPMX19_00056 Leucine--tRNA ligase (Azospirillum sp. SherDot2)
MSRYNVKETEAKWQGVWDSKGCFTAREDASRPKYYVLEMFPYPSGRIHMGHVRNYTIGDV
IARFKRAKGFNVLHPMGWDAFGLPAENAALEKKVHPAAWTRENIATMRGQLKTMGLSIDW
DREIATCDVEYYRHEQKMFLDFLKAGLAYRKESWVNWDPVDNTVLANEQVIDGRGWRTGA
LVEKRKLSQWFLKITAYAEDLLKGLETLDRWPERVRIMQENWIGKSTGVRFRFKIKGRED
DLEVFTTRPDTLFGASFAAISPNHPLAAELAASSPELAEFIAECNRLGTSEEVIETAEKR
GFDTGLTVMHPFDPSWELPVYVANFVLMEYGTGAIFACPAHDQRDLDFARKYGLPVRPVV
IPADADPAAFEVGTEAYTGPGVLRNSAFLDGLDTESAKEEAGKRLEAAEQGERTTQYRLR
DWGVSRQRYWGCPIPVIHCESCGIVPVPDDQLPVVLPEDVTFDKPGNPLDHHPTWKHTGC
PQCGKPALRETDTFDTFIESSWYFARFCSPKTENAAFTHEAVDYWLGVDQYIGGIEHAVL
HLLYSRFWTRALKTCGYLNLDEPFTGLFTQGMVNHETYKDTGTGAWLAPTDLTKNDKGDW
VRADTGAPVNVGRVEKMSKSKKNVVDPAHIIGTYGADAARLFMLSDSPPERDLEWTEAGI
DGAWRYINRLWRMMTEADLPAAGAPKPESLGPRAEAARRLVHKTIAGVSEDLDKFRFNKA
VARVRELSNALGELDGKGEGEGWVLREGFESLVRLVGPMMPHLGEELWAQLGHGSLLADQ
PWPEADPALVVEDSVKVAVQVNGKLRATLELPRDMDKDAAEQAALADVNVQRAMDGKPAR
KVIVVPNRVINVVV