Protein Info for MIT1002_04027 in Alteromonas macleodii MIT1002

Annotation: Protoheme IX farnesyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 transmembrane" amino acids 31 to 48 (18 residues), see Phobius details amino acids 54 to 77 (24 residues), see Phobius details amino acids 98 to 121 (24 residues), see Phobius details amino acids 127 to 145 (19 residues), see Phobius details amino acids 152 to 171 (20 residues), see Phobius details amino acids 177 to 199 (23 residues), see Phobius details amino acids 220 to 242 (23 residues), see Phobius details amino acids 248 to 266 (19 residues), see Phobius details amino acids 281 to 301 (21 residues), see Phobius details TIGR01473: protoheme IX farnesyltransferase" amino acids 21 to 299 (279 residues), 334.2 bits, see alignment E=4.4e-104 PF01040: UbiA" amino acids 35 to 283 (249 residues), 232 bits, see alignment E=3.6e-73

Best Hits

Swiss-Prot: 75% identical to CYOE2_PSEA6: Protoheme IX farnesyltransferase 2 (cyoE2) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K02301, protoheme IX farnesyltransferase [EC: 2.5.1.-] (inferred from 94% identity to amc:MADE_04005)

MetaCyc: 36% identical to heme O synthase (Escherichia coli K-12 substr. MG1655)
HEMEOSYN-RXN [EC: 2.5.1.141]

Predicted SEED Role

"Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone" in subsystem Biogenesis of cytochrome c oxidases

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.- or 2.5.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (305 amino acids)

>MIT1002_04027 Protoheme IX farnesyltransferase (Alteromonas macleodii MIT1002)
MAKSVSLHARTSRQHNVVTWRDYYEMTKPNVVMLLLLTALVGMCLATPSWVNATVLICGL
LGIGMLSASAAVINHVVDHKIDSVMARTFNRPVAKGKVSVNNALWFAGVLGVVGFLVLAL
FVNMLTAWLTLASLVGYAFIYTMYLKRATPQNIVIGGLAGAAPPLLGWTAVTGEIHAHAL
LLVLIIFTWTPPHFWALAIHREKDYAKAKVPMLPVTHGVSFTKTSVLLYTVLLGLVCLLP
YLTGMSDLIYLIGSSVLNIGFLYYALKLKFDAEPQTAMKTFRFSIIHLMVLFVVLLLDHY
IPLSL