Protein Info for MIT1002_03665 in Alteromonas macleodii MIT1002

Annotation: Primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729 PF17764: PriA_3primeBD" amino acids 5 to 101 (97 residues), 93.2 bits, see alignment E=2.3e-30 PF04851: ResIII" amino acids 203 to 359 (157 residues), 47.1 bits, see alignment E=7.5e-16 PF00270: DEAD" amino acids 203 to 365 (163 residues), 69.4 bits, see alignment E=9.8e-23 TIGR00595: primosomal protein N'" amino acids 221 to 725 (505 residues), 591.2 bits, see alignment E=8.4e-182 PF18319: PriA_CRR" amino acids 445 to 467 (23 residues), 32.3 bits, see alignment (E = 2.3e-11) PF00271: Helicase_C" amino acids 504 to 588 (85 residues), 27.6 bits, see alignment E=9.1e-10 PF18074: PriA_C" amino acids 632 to 725 (94 residues), 64 bits, see alignment E=5.8e-21

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 92% identity to amc:MADE_03519)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (729 amino acids)

>MIT1002_03665 Primosomal protein N' (Alteromonas macleodii MIT1002)
MAFIQVAVPVPLRQLFTYTHDNALSAGVRVVVPFGPRKLVGVVVETLHKPESDIENSNKL
KAIESVLDDSPIIDGVLLKMAQWLWQYYHHAPGEVLHAMLPVLLRKGESAAPTPQDMLML
TEEGKQTTADTLSRAPKQQACFTAMAGGALLASQARKQYGAPAVKALVEKSLISIEEVAP
EFKSGSWLASLSVSEKPIPDTEQSVAIAALNRQQGNFAVSLLEGVTGSGKTEVYLQAIEP
LLRDGKQVLILVPEIGLTPQTVSRFEKRFGIAVGVLHSQLSDKERLHVWQRAKAGELGII
IGTRSAIFTPLHNPGMLIVDEEHDESFKQQDGLRYHARDLAAMRAKQHNIPLLLGSATPA
LETLNNALSGRYAHLQLTKRAGGAKSTHQHVLDARDQPIHYGISQGLLTIMRQHINAGNQ
VLVFVNRRGYAPALLCHHCGETVMCKRCDRPYTVHKAQNRLQCHHCGGMRSMPSNCEACH
HNELVTAGTGTEQVEQGLASLFPGVKQVRIDSDTVRGKDKLHQTLDAINRQEYQLLVGTQ
ILSKGHHFPHVTLVAVLDCDGALFSADFRAPEKLAQLVTQLAGRAGRASKPGEMWLQTHN
PHHPLLQDLVHNGYGHFARHALMERKAAGLPPFISQFVIRAEATDSSLAYRFLQDSKQVF
LQQHAIELNGPFPCLIEKRQGRFRFMLVCSHEKRAPLHNALRLALPFLQALPQAMKVRWS
IDIDPTDFS