Protein Info for MIT1002_03539 in Alteromonas macleodii MIT1002

Annotation: Bifunctional protein BirA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08279: HTH_11
  • amino acids 9 to 61 (53 residues), 40.3 bits, 3.5e-14
• TIGR00121: biotin--[acetyl-CoA-carboxylase] ligase
  • amino acids 87 to 326 (240 residues), 191.8 bits, E=7.1e-61
• PF03099: BPL_LplA_LipB
  • amino acids 91 to 217 (127 residues), 84.9 bits, E=7.1e-28
• PF02237: BPL_C
  • amino acids 283 to 326 (44 residues), 32.6 bits, 9.5e-12

Best Hits

KEGG orthology group: K03524, BirA family transcriptional regulator, biotin operon repressor / biotin-[acetyl-CoA-carboxylase] ligase [EC: 6.3.4.15] (inferred from 100% identity to amc:MADE_03573)

Predicted SEED Role

"Biotin--protein ligase (EC 6.3.4.9, EC 6.3.4.10, EC 6.3.4.11, EC 6.3.4.15) / Biotin operon repressor" in subsystem Biotin biosynthesis

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• 3-hydroxypropanoate cycle (6/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.15

Protein Sequence (328 amino acids)

>MIT1002_03539 Bifunctional protein BirA (Alteromonas macleodii MIT1002)
MRQASKAIRKHILALLSDGQFHSGETIAQQVGLSRTAVAGHISQLADWGLDVFKVKGKGY
RLERGFPLLSADSIKRHLGNTKRAVIDVESVLPSTNTEMKKRIASKREDLENGDVVFAEI
QTDGRGRHGRSWLAPVGGSLTFSMYWSFPDGYQSMAGLSLLVGLAVCDALKEFGVEDAEL
KWPNDIYLQGKKLAGVLIEVEGQIGATAHSVIGIGLNVCVPDNQYDVGQPHSDLASYLGT
IPDRNALAAEIIKSLWSYLPKFTQQGFGPFTAYWEALDLYADKRIVLQMGEKRFSGIDRG
VDASGALLVETQDGITRFHGGEVSLRGN