Protein Info for MIT1002_03483 in Alteromonas macleodii MIT1002

Annotation: Na(+)-translocating NADH-quinone reductase subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details TIGR01941: NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit" amino acids 6 to 410 (405 residues), 739.3 bits, see alignment E=5.5e-227 PF00111: Fer2" amino acids 40 to 117 (78 residues), 34.4 bits, see alignment E=2.5e-12 PF00970: FAD_binding_6" amino acids 204 to 269 (66 residues), 36.5 bits, see alignment E=7.7e-13 PF00175: NAD_binding_1" amino acids 280 to 388 (109 residues), 65.5 bits, see alignment E=9.8e-22

Best Hits

Swiss-Prot: 89% identical to NQRF_PSEA6: Na(+)-translocating NADH-quinone reductase subunit F (nqrF) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00351, Na+-transporting NADH:ubiquinone oxidoreductase subunit F [EC: 1.6.5.-] (inferred from 100% identity to amc:MADE_00750)

MetaCyc: 82% identical to Na(+)-translocating NADH-quinone reductase subunit F (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit F (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (410 amino acids)

>MIT1002_03483 Na(+)-translocating NADH-quinone reductase subunit F (Alteromonas macleodii MIT1002)
MNNVEIFLGVGMFIAIVLALVFIIMFAKSKLVPSGDVTITINGDPDKAIKTAPGGKLLGA
LADAGYFVSSACGGGGSCGQCRVDVHSGGGEILPTELDHITKGEAREGCRLSCQVAIKQD
MEIELEESVFGVKKWDCEVISNDNKATFIKELKLKIPNGESVPFRAGGYIQIEAPAHHVK
YKEFDIPEEYRGDWERFGFFDIESKVDEETIRAYSMANYPEEEGIIMLNVRIATPPPNNL
SLPAGKMSSYIWSLKEGDKATISGPFGEFFAKETENEMVFVGGGAGMAPMRSHIFDQLRR
LKSKRKMSFWYGARSLREMFYTEDFDELAAENDNFEWHVALSDPQPEDNWEGYTGFIHQV
LLENYLKDHPAPEDCEFYMCGPPMMNAAVINMLKDLGVEDENIMLDDFGG