Protein Info for MIT1002_03395 in Alteromonas macleodii MIT1002

Annotation: Type-1 restriction enzyme R protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1150 PF13643: DUF4145" amino acids 23 to 112 (90 residues), 23.5 bits, see alignment 1.8e-08 PF04313: HSDR_N" amino acids 205 to 367 (163 residues), 26.9 bits, see alignment E=1.6e-09 PF04851: ResIII" amino acids 425 to 613 (189 residues), 117.2 bits, see alignment E=2.9e-37 PF00270: DEAD" amino acids 429 to 569 (141 residues), 32.9 bits, see alignment E=2.1e-11 PF18766: SWI2_SNF2" amino acids 431 to 641 (211 residues), 49 bits, see alignment E=2.5e-16 PF00271: Helicase_C" amino acids 716 to 819 (104 residues), 33.3 bits, see alignment E=2.1e-11 PF08463: EcoEI_R_C" amino acids 973 to 1135 (163 residues), 112.4 bits, see alignment E=9.8e-36

Best Hits

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1150 amino acids)

>MIT1002_03395 Type-1 restriction enzyme R protein (Alteromonas macleodii MIT1002)
MVFTSNFEFLKAHGVWFYNLAASAERNFSSDPNTTLIKMRQLGEAIAHNIAARVGVEFGK
EVTQHNLLKDLDYTLRLESNVREAFYDLKSLGNSATHEFDSSTHRDALKALIIGHSLASW
FHSTFGGEKAKGFNKSKFVKPEDPSDYVRQLEEKLSQITIESQRTSDRIKVAEKLSQIEH
DKAEAERLRAERMAEEREIWEQLAHEQEEALANYRAEVERENKVNFDHFKAQPKEQQAAE
LQRIEKSHFEMDEAETRVIIDQQLIDAGWEADTENLRYSKGARPEPNKNRAIAEWPTDSG
PADYALFIGTTLVAVVEAKKNARNVYGAIDQAKRYAKGISSLADNIEVTEYGEFKVPLTF
ATNGRAYLKQLEQESGIWFLDVRDTTNRRKALKGWYSPQKIKTYLRQTPQKANAQLDEMG
FEYELQLRDYQKKAIQSVEQAIKDEKERALVAMATGTGKTKTCIALVYRLLKAERFRRIL
FLVDRSALGVQATIDFGEVRMENLNTFADTFDVLGMEPEKNPKPTPDDDTKVHVATVQGL
VKRIMYPSEDATKPGVGQYDCIVVDECHRGYLLDRELSETEVKFRDQQDYVSKYRAVIDY
FDAFKIGLTATPAIHTTDIFGDPVFSYSYTEAVIDGYLVDHLPPVRIHTKLNTDGIHYNV
NEEVQVYDPQTNQLELIHTPDELDFDVAQFNRKVIAPEFTKAIAKWLIESDSIDPYSQEK
TLIFCVTDKHADEMVEALKDACEHYHGAVDDDAIMKITGASDKPLQLIRRYKNDRLPNIV
VTVDLLTTGVDVPSICNLVFMRRVNSRILYEQMLGRATRRCDDIAKERFRIYDAVDIYKQ
LEKVNTMKPVVTNVDITFSKLESEIKEGKDSKLQELARDQFLSKLQSKKRYLTESQQSEF
ERIVGKPPQEFAQELKAKPVHEVADWFLNNPGLGELLDMKVKGTGGFTRIVISEHEDEVL
DTSTGYGSGQKPEDYLTAFNDFVNANSNRMVAIQTVIQRPWELTRESLKQLALELEKNEF
REEDLKAAWKEVRNEDIAARIIGYIRQAAIGEALIPYEQRVDRALDRILASQPWKTPQRD
WLSTIAKQMKANIIVDEANLNEGIFKQRLGGINRANRLFDQPITDILAQFNRALWVNDDD
GGYTDDKATA