Protein Info for MIT1002_03331 in Alteromonas macleodii MIT1002
Annotation: D-lactate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to DLD_ECOLI: Quinone-dependent D-lactate dehydrogenase (dld) from Escherichia coli (strain K12)
KEGG orthology group: K03777, D-lactate dehydrogenase [EC: 1.1.1.28] (inferred from 96% identity to amc:MADE_01510)MetaCyc: 57% identical to quinone-dependent D-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
DLACTDEHYDROGFAD-RXN [EC: 1.1.5.12]
Predicted SEED Role
"D-Lactate dehydrogenase (EC 1.1.2.5)" in subsystem Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.5)
MetaCyc Pathways
- heterolactic fermentation (15/18 steps found)
- superpathway of glucose and xylose degradation (14/17 steps found)
- mixed acid fermentation (13/16 steps found)
- L-alanine degradation II (to D-lactate) (3/3 steps found)
- D-lactate to cytochrome bo oxidase electron transfer (2/2 steps found)
- pyruvate fermentation to (R)-lactate (1/1 steps found)
- 2-chloroacrylate degradation I (2/3 steps found)
- methylglyoxal degradation I (2/3 steps found)
- superpathway of methylglyoxal degradation (5/8 steps found)
- vancomycin resistance I (1/3 steps found)
- superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
- lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) (4/8 steps found)
- L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
- superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.28, 1.1.2.5
Use Curated BLAST to search for 1.1.1.28 or 1.1.2.5 or 1.1.5.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (577 amino acids)
>MIT1002_03331 D-lactate dehydrogenase (Alteromonas macleodii MIT1002) MTVNVDTQSLIASMRDVVGQAYLLTDKAKKQPYTKGFRFGVGEALAVVRPGTLVEIWRVL KLCVEADVAVIMQAANTGLTGGSTPDGKDYDRPLVIISTMRIDDIQLVDNGKQIVGLAGS TLFGLEETLAPYGREPHSVIGSSCIGASIVGGICNNSGGALVKRGPAYTELALFAQIDVN GNLSLVNNLGINLGSEPEEILNNLENKRYKEVDVQHPDARASDNEYDERVRDVDSDTPSR FNNDGRRLHEASGCAGKLAVFAVRLDTFPIPEKKQVFYVGSNDAAVFTKVRRDILSTFKN LPDSGEYLHRDCYDVSKKYGKDMFIVISKLGAKFIPKLFAFKRKFDAFTDKLGFLPNKMS DRVMQYMSYVFPNHLPKRMEEYRDKYQHHWILEMSNDGVDEAKAYLDAFFKTNEGSYFEC TEEEADKAILHRFVAGGAIGRYHVMHSKDVGAMMTIDVALRRNDPDWFEVLPKEIDDQID TKLYYGHLFCHVMHQNYVLKKGADAKLLKGKILETFDARSAEYPAEHNVGHEYFAKDALK NFYRELDPTNSFNPGIGKTTKLKNWAEHDGSCCGGHH