Protein Info for MIT1002_03326 in Alteromonas macleodii MIT1002

Annotation: Methylmalonate-semialdehyde dehydrogenase [acylating]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 5 to 482 (478 residues), 622.7 bits, E=2.1e-191
• PF00171: Aldedh
  • amino acids 15 to 478 (464 residues), 446.3 bits, E=1.1e-137
• PF05893: LuxC
  • amino acids 133 to 268 (136 residues), 24.4 bits, E=1.3e-09

Best Hits

Swiss-Prot: 53% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 98% identity to amc:MADE_01513)

MetaCyc: 44% identical to (methyl)malonate-semialdehyde dehydrogenase monomer (Bacillus subtilis subtilis 168)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]; Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.27, 1.2.1.18]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• β-alanine degradation I (1/2 steps found)
• β-alanine degradation II (1/2 steps found)
• L-valine degradation I (4/8 steps found)
• acrylate degradation I (1/5 steps found)
• propanoyl-CoA degradation II (1/5 steps found)
• 2,4-dinitrotoluene degradation (1/7 steps found)
• myo-inositol degradation I (1/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27

Protein Sequence (499 amino acids)

>MIT1002_03326 Methylmalonate-semialdehyde dehydrogenase [acylating] (Alteromonas macleodii MIT1002)
MRKIVHFIAGKDFEDSSENTASVFAPNTGEVQAEVSLANTSTLAYAIETAQAAQVEWAAT
NPQRRARVMFKFKELVEANRDRLAALLSSEHGKVLVDAQGDIQRGLEVIEFACGIPHAQK
GEYTEGAGPGIDVYSMRQALGVVAGITPFNFPAMIPMWMFGVAIACGNAFILKPSERDPS
VPVELAKLMIEAGLPAGVLNVVHGNKEVVDGILDHQTIKAVSFVGSSDIANYVYERGARA
GKRVQAMGGAKNHGIVMPDADLDQVVTDLVGAAYGSAGERCMALPVVVPVGDETAEKLKA
KLIPAIKDIMVSHSEDPNAHYGPVVTAAHKERVEGYIDLCIEEGAELVYDGRTSQIPDDS
QGYFIGPTFFDHVTKDMRSYQEEIFGPVLQMVRAKDFNEAVNLASDHQYGNGVAMYTRNG
HAAREFAQRVNVGMVGINVPIPVPVAYHTFGGWKRSAFGDTNQHGMEGVKFWTKVKTITQ
RWPDGSDTNDNAFVIPTMG