Protein Info for MIT1002_03250 in Alteromonas macleodii MIT1002

Annotation: Glucose--fructose oxidoreductase precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF01408: GFO_IDH_MocA" amino acids 35 to 158 (124 residues), 92.7 bits, see alignment E=4.3e-30 PF22725: GFO_IDH_MocA_C3" amino acids 167 to 287 (121 residues), 65.4 bits, see alignment E=7.4e-22 PF02894: GFO_IDH_MocA_C" amino acids 191 to 362 (172 residues), 42.8 bits, see alignment E=8.6e-15

Best Hits

KEGG orthology group: K00118, glucose-fructose oxidoreductase [EC: 1.1.99.28] (inferred from 88% identity to amc:MADE_01557)

Predicted SEED Role

"Glucose-fructose oxidoreductase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.99.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (362 amino acids)

>MIT1002_03250 Glucose--fructose oxidoreductase precursor (Alteromonas macleodii MIT1002)
MPRITRRHFLQLAGSGLLVAQSPYLMAKPSKKKIGVALLGLGNYSTNLLAPALQDTKHCE
LRGIITGSENKIPQWQQKYGIKDSNVYTYSTMADIANNDDIDVIYVVTPTGTHEDFAVQA
ANTGKHVWCEKPMAMDSEECQAIIDACDKNGVQLSIGYRMQHEPNTRTFHQYLETKPYGN
LTAVSSFAGYAGQGRAPDNWRMKKHMGGGALYDMGVYAINGARFLTGREPLAVTGSHPPQ
RFPETFTEVDETTLFTMDFGDGLVADCGTSVVEGFNHFKADCEKGWYQLKPMQSYSGVTG
TTSDGKTLAPINGMQQTLQMDNDALAILTGRQPMVTGNEGLADIRIVNAIFKAAKTGETV
AL