Protein Info for MIT1002_03195 in Alteromonas macleodii MIT1002

Annotation: Valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 924 transmembrane" amino acids 473 to 494 (22 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 905 (905 residues), 1228.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 13 to 599 (587 residues), 491.6 bits, see alignment E=4.5e-151 PF09334: tRNA-synt_1g" amino acids 40 to 90 (51 residues), 25 bits, see alignment 2e-09 amino acids 330 to 418 (89 residues), 22.1 bits, see alignment E=1.6e-08 PF13603: tRNA-synt_1_2" amino acids 238 to 297 (60 residues), 35.2 bits, see alignment 2.3e-12 PF08264: Anticodon_1" amino acids 642 to 797 (156 residues), 145.3 bits, see alignment E=3.3e-46 PF10458: Val_tRNA-synt_C" amino acids 859 to 921 (63 residues), 53.2 bits, see alignment 7.6e-18

Best Hits

Swiss-Prot: 65% identical to SYV_ALCBS: Valine--tRNA ligase (valS) from Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 98% identity to amc:MADE_01578)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (924 amino acids)

>MIT1002_03195 Valine--tRNA ligase (Alteromonas macleodii MIT1002)
MDKTFEPQSIEQQCYKSWEEAGLFKASGSGDPYCILLPPPNVTGSLHMGHGFQQTIMDAL
TRYHRMKGDNTLWQVGTDHAGIATQMVVERQLNAQGKTRHDLGREDFIKKVWEWKEHSGG
TITGQMRRLGTSPDWSREVFTMDEDLSKAVTEVFVKLHEEGLIYRGKRLVNWDPVLHTAV
SDLEVLNEEEDGHMWHMRYPLADGSGELVVATTRPETMLGDTAVAVHPDDERYQDFIGKE
IKLPITGRLIPVIADDYVDQEFGTGCVKITPAHDFNDYDMGKRHNLPMINILTDDAKIND
DAPEAYRGLDRFDARKQIVADLDAQGALVKIEPHKLKVPRGDRTGAVIEPYLTDQWYVAV
ESLAKPAIEAVESGEIRFVPENWNKTYYQWMHNIQDWCISRQLWWGHRIPAWYDENGNVF
VGRTEEEVREKHGLGSDVTLSQDDDVLDTWFSSALWPFATMGWPEETPDLETFVPSSVLV
TGFDIIFFWVARMIMMTKKFTGKIPFKDIYITGLIRDESGDKMSKSKGNVLDPIDLIDGI
DIESLVTKRTAGMMQPQLAEKIAKRTRKQFPDGIQAYGTDALRFTFAAMASTSRDINFDM
ARVEGYRNFCNKIWNASRFVLMNTEEHDTGRDGGEMVLSMADRWIWAKFQQTLVEFEKAL
EDYRFDIAAQTVYEFTWNQFCDWYLELTKPVLNNDASTEVEKRGTRHTLINVLESLLRLL
HPLMPFITDTIWQRVVPLSALKVEEGASIMVQAFPEVDAAKQDDKVLADIEWVKKFIVGI
RNIRGEMDISPNKPLNALLKNVSDEDARRLDAAKAFLDKLSKLETVTILKDGEEAPASAT
ALVGEMEILIPMAGLIDKDAELARITKAMEKIEKDVSRTRGKLGNEKFVSNAPEAVIEKE
RAKLEEGEKALAKLKEQFETIKAL