Protein Info for MIT1002_03185 in Alteromonas macleodii MIT1002

Annotation: Nitrogen regulation protein NR(II)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 PF00989: PAS" amino acids 8 to 106 (99 residues), 39.8 bits, see alignment E=1e-13 PF08448: PAS_4" amino acids 13 to 88 (76 residues), 30.9 bits, see alignment E=7.1e-11 PF13426: PAS_9" amino acids 18 to 107 (90 residues), 28 bits, see alignment E=5.6e-10 PF00512: HisKA" amino acids 134 to 188 (55 residues), 56.5 bits, see alignment 5.7e-19 PF02518: HATPase_c" amino acids 236 to 353 (118 residues), 68.1 bits, see alignment E=2.2e-22

Best Hits

KEGG orthology group: K07708, two-component system, NtrC family, nitrogen regulation sensor histidine kinase GlnL [EC: 2.7.13.3] (inferred from 96% identity to amc:MADE_01589)

Predicted SEED Role

"Nitrogen regulation protein NtrB (EC 2.7.13.3)" (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (361 amino acids)

>MIT1002_03185 Nitrogen regulation protein NR(II) (Alteromonas macleodii MIT1002)
MHSTELETQQLINSLNTALVMVDNKLTIVYANHAGEALFETGLKQLIGHPLQDFFLPGTI
ETTRLKAAIRKGEDFTENEIKLAFRDNRYVSADLTVTNLHFEDGPRLLFEVKKIDQQKRI
SRETLQNAQHYAARELVRGLAHEIKNPLGGIRGAAQLLEKELIDEHKEFTSMIIEQSDRL
RNLVDRLLGPNSLPRFEESNVHQALEKIRSLMRFDSDNPITIVRDYDPSIPPLMLDPDMI
QQAVLNIVRNSVQALTESQTPNPQIRLITRIERQMTIQTKRHALVAKIKIIDNGPGIPAE
VKDTLFYPMVTSKQNGSGLGLSISQTLINHHGGKIDVDSYPGHTEFVIYIPIDRKETDDD
E