Protein Info for MIT1002_03143 in Alteromonas macleodii MIT1002

Annotation: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 3 to 229 (227 residues), 178.6 bits, E=6.7e-57
• TIGR01704: MTA/SAH nucleosidase
  • amino acids 3 to 230 (228 residues), 267.7 bits, E=3.9e-84

Best Hits

Swiss-Prot: 52% identical to MTNN_VIBPA: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 95% identity to amc:MADE_01627)

MetaCyc: 52% identical to 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase monomer (Vibrio cholerae)
Methylthioadenosine nucleosidase. [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (4/6 steps found)
• S-methyl-5'-thioadenosine degradation I (1/2 steps found)
• autoinducer AI-2 biosynthesis I (3/5 steps found)
• 5'-deoxyadenosine degradation II (1/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• L-methionine salvage cycle II (plants) (2/11 steps found)
• L-methionine salvage cycle I (bacteria and plants) (2/12 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.16, 3.2.2.9

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Protein Sequence (233 amino acids)

>MIT1002_03143 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Alteromonas macleodii MIT1002)
MKKIGILGAMDEEVALLKASLWNVKETQWKHLTFYEGTLNDIEVVLVKCGIGKVAAAIAT
TVLIEQYAPDAVVNTGSAGGFDKNLNIGDLVIASHVIHHDADLTHFGYKLGQCAGMPEDF
RCDTRLMEAARSAATGITSLQSTTGLICTGDAFIGSDEAVAELRENFPDMKAVEMEGAAI
GQTCHMLDTPFLVIRSLSDIAGKTSSVSFKEYLDTAAKHSAQLVMAMIKALAK