Protein Info for MIT1002_03132 in Alteromonas macleodii MIT1002

Annotation: 3-methylitaconate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 transmembrane" amino acids 313 to 336 (24 residues), see Phobius details TIGR02334: probable AcnD-accessory protein PrpF" amino acids 6 to 391 (386 residues), 720.6 bits, see alignment E=2.1e-221 PF04303: PrpF" amino acids 7 to 389 (383 residues), 600.5 bits, see alignment E=6.4e-185

Best Hits

Swiss-Prot: 76% identical to PRPF_SHEON: 2-methyl-aconitate isomerase (prpF) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K09788, hypothetical protein (inferred from 92% identity to amc:MADE_03373)

Predicted SEED Role

"2-methylaconitate cis-trans isomerase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (393 amino acids)

>MIT1002_03132 3-methylitaconate isomerase (Alteromonas macleodii MIT1002)
MTKYAPQLRVPATYMRGGTSKGVFFNLTDLPEAAQKPGPARDALLLRVIGSPDPYGKQTD
GMGGATSSTSKTVILSKSEREGYDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAITNG
LVDRSKLPENGIATVNIWQANINKAIIARVPMTNGEVQETGDFELDGVTFPAAEVEVAFV
DPADGEGAIFPTGRLVDTLEVPGVGSLQATMINAGIPTIFINAEDIGYTGTELQDDINSD
SEALSKFEAIRAHGAVQMGLINDVGEAANRQHTPKVAFVAPPRGYQASSGKDILGSDIDV
LVRALSMGKLHHAMMGTAAVAIAAAAAIPGTLVNIAAGGDERDSVTFGHPSGTLRVGARA
TFEDDKWQIKQAVMSRSARILMEGSVRVPAEQS