Protein Info for MIT1002_03096 in Alteromonas macleodii MIT1002

Annotation: Isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 PF20423: AceK_regulatory" amino acids 17 to 316 (300 residues), 339.2 bits, see alignment E=2.1e-105 PF06315: AceK_kinase" amino acids 317 to 568 (252 residues), 361.9 bits, see alignment E=2.1e-112

Best Hits

Swiss-Prot: 67% identical to ACEK_PSEA6: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 96% identity to amc:MADE_03338)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (576 amino acids)

>MIT1002_03096 Isocitrate dehydrogenase kinase/phosphatase (Alteromonas macleodii MIT1002)
MQELATSQDKSLVRKVAYQILSHFDKSYRWFTRITRGAQERFEKGNWKETQLASKERITI
YEQSLSDAVAEIYQLTQVHQKDDAFWQELKKVFALQLEGHPQFELAETFYNSVIGRLFKH
RKIDNDMMFVLPSRCFLPGQDRDKVINSFDTTTTVREMYESIFKIYRYNIPFENFERDLQ
NLESALRARLNKEQLASVQAVEILKPTFFRGKAAYLIGRICMPDETLPFVIAMRRYNEPH
MFVDALLTDRKDLSVIFGFARSYFMADTQNPAEVAAFLQELLPNKKHFELYMAMGHYKHG
KTVFYRNFLQHMDESDDKFEAAPGIRGLVMMVFHLPSYGVVFKIIKDEFAESKKITREHV
KECYKLVKMSDRVGRMADTHEYVNFRFPLDRIEPELIEELKETCASSLEFTESELIIKHM
YIERKMTPLNLYLQEETDEEKITRALDELGLCIKQIAMANIFPGDMLHKNFGITRHGRVI
FYDYDEICLMNERNFRSLPKSDDPYAIDTLSVAPNDVFPEQFEHFIVGKRKFKDILKSLH
GDLMTPEYWHEVQEKCARGDVQHFTPYNASMRFDRG