Protein Info for MIT1002_03083 in Alteromonas macleodii MIT1002

Annotation: Uracil-DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 TIGR00628: uracil-DNA glycosylase" amino acids 6 to 215 (210 residues), 289.5 bits, see alignment E=8.4e-91 PF03167: UDG" amino acids 50 to 211 (162 residues), 71.1 bits, see alignment E=5.5e-24

Best Hits

Swiss-Prot: 71% identical to UNG_VIBPA: Uracil-DNA glycosylase (ung) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K03648, uracil-DNA glycosylase [EC: 3.2.2.-] (inferred from 90% identity to amc:MADE_03325)

MetaCyc: 65% identical to uracil-DNA glycosylase (Escherichia coli K-12 substr. MG1655)
RXN0-2584 [EC: 3.2.2.27]

Predicted SEED Role

"Uracil-DNA glycosylase, family 1" in subsystem DNA Repair Base Excision

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.- or 3.2.2.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (223 amino acids)

>MIT1002_03083 Uracil-DNA glycosylase (Alteromonas macleodii MIT1002)
MTPSATTWQSALGDEKQKDYFVTLLERVKHAREAGSVIYPPLSDVFNALKYTPLDEVKVV
ILGQDPYHGPNQAHGLCFSVKAGVKTPPSLKNMYKELLSDIDGFTEPNHGTLTSWAEQGV
LLLNTVLTVEQGKAHSHAKWGWETFTDKVIDVVNEHCQNVVFLLWGSHAQKKGKHINREK
HHVLHAPHPSPLSAHRGFLGCKHFSQTNEYLIAKGKEPINWQV