Protein Info for MIT1002_03055 in Alteromonas macleodii MIT1002

Annotation: Glutathione synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 PF02951: GSH-S_N" amino acids 5 to 121 (117 residues), 141.4 bits, see alignment E=2.8e-45 TIGR01380: glutathione synthase" amino acids 5 to 313 (309 residues), 413 bits, see alignment E=3.3e-128 PF02955: GSH-S_ATP" amino acids 125 to 297 (173 residues), 243.1 bits, see alignment E=2.6e-76 PF14398: ATPgrasp_YheCD" amino acids 132 to 187 (56 residues), 27 bits, see alignment E=5.5e-10 PF08443: RimK" amino acids 146 to 310 (165 residues), 42.4 bits, see alignment E=1.2e-14

Best Hits

Swiss-Prot: 61% identical to GSHB_VIBCH: Glutathione synthetase (gshB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 96% identity to amc:MADE_03292)

MetaCyc: 61% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.3

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (317 amino acids)

>MIT1002_03055 Glutathione synthetase (Alteromonas macleodii MIT1002)
MQYTVGVIMDPIASIKPHKDTSFAMMLEAQRRGAKVVYFELKDLYLDNGKPMGLGKTVSV
KDQATDFYTIEDESTLYLGDVDVLLMRKDPPFDSEFLYATQVLSLAQDAGALVVNNPQAL
RDYNEKLFTSYFPEHIPNTLVTNNQALIREFHAKHQDIICKPLDGMGGASIFRVKPDGNN
LGVIIETLTKLGTRYMMVQEYLPEIKDGDKRVLIVDGEVMPYCLARLPTKGETRGNLAAG
GTGRPQPISDSDRALAEAIAPTLKENNILFVGLDVIGSKITEINITSPTCVREIEAAYDV
NIMGALFDAIEKRLASK