Protein Info for MIT1002_03039 in Alteromonas macleodii MIT1002

Annotation: Cation efflux system protein CusB precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 587 transmembrane" amino acids 16 to 38 (23 residues), see Phobius details PF19335: HMBD" amino acids 74 to 100 (27 residues), 31 bits, see alignment (E = 1e-10) PF25973: BSH_CzcB" amino acids 158 to 275 (118 residues), 34.9 bits, see alignment E=5.8e-12 PF25919: BSH_CusB" amino acids 159 to 275 (117 residues), 73.2 bits, see alignment E=6e-24 PF25869: 3HB_CusB" amino acids 194 to 241 (48 residues), 46.1 bits, see alignment 1.7e-15 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 242 to 424 (183 residues), 102.7 bits, see alignment E=9.5e-34 PF25954: Beta-barrel_RND_2" amino acids 279 to 357 (79 residues), 64.2 bits, see alignment E=5.2e-21 PF25967: RND-MFP_C" amino acids 364 to 420 (57 residues), 28.7 bits, see alignment 5.1e-10 PF25975: CzcB_C" amino acids 367 to 425 (59 residues), 34.3 bits, see alignment 9.4e-12 PF11604: CusF_Ec" amino acids 468 to 520 (53 residues), 41.6 bits, see alignment 4.6e-14

Best Hits

KEGG orthology group: K07798, Cu(I)/Ag(I) efflux system membrane protein CusB (inferred from 49% identity to psm:PSM_A2788)

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (587 amino acids)

>MIT1002_03039 Cation efflux system protein CusB precursor (Alteromonas macleodii MIT1002)
MTSSTHPAPDTKSTLIKGLLVGLLFGGLIAFGLASLLLSDSSKHGNSESAHSGVSAHSDK
SAHSDETDNKAPLYWVAPMDDSYRRDKPGKSPMGMDLVPVYANDDATSAKSNERTPGTVM
IPPNVQHNIGVKVAPVTIGALQQTVTAVGNVAYDEDSIVHIHPRVSGWVDRLFIKSQGEQ
VEEGQALYTLYSPELVSAQEEYVLALKRGDARLIQGAAERLRTLGLSTSVIEALQKTRRV
TQTVTFNAPQSGVVEALNIREGFYVQPGTTLMSIAQLDTVWVIAEVPEKYAQIIAPHNGA
VITLDEMDHGSDAKWESHVEYIYPSLSETTRTLKVRIPLNNNNHTLKPNMFAHVGIKPTQ
RKAALLVPRSAVIRTAEGAKVVLSDGDGNFKSVRVTLGQSDSTHLEVVEGLLPEDDVVVS
AQFLIDSESSKSSDFERMKAPDHQATTTGTIEAIALPADTETNGASNDAKLVIARGPIEK
WGRGPATMSFSVSKHIDLSQFKEGDNIMFTFVTGEEFTVIDMRKRSEHAMDKGMVHGTEH
SNHKHDTSNLKGDGEQSDAHESIVHKSMVHKSAEQKSINHKEHMHHD